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MABD122A4

Sigma-Aldrich

Anti-CD73, clone AA60-E3-3, Alexa Fluor 488 conjugate Antibody

clone AA60-E3-3, from mouse, ALEXA FLUOR 488

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Synonym(s):
CD-73, 5′-nucleotidase, 5′-NT, ecto-5′-nucleotidase
eCl@ss:
32160702
NACRES:
NA.44

biological source

mouse

Quality Level

conjugate

ALEXA FLUOR 488

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AA60-E3-3, monoclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (immunogen homology)

technique(s)

immunocytochemistry: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NT5E(4907)

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This Item
MABD122SAB4701116SAB4700863
antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

AA60-E3-3, monoclonal

clone

AA60-E3-3, monoclonal

clone

AD2, monoclonal

clone

AD2, monoclonal

species reactivity

human

species reactivity

human

species reactivity

-

species reactivity

human

Gene Information

human ... NT5E(4907)

Gene Information

human ... NT5E(4907)

Gene Information

human ... NT5E(4907)

Gene Information

human ... NT5E(4907)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

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General description

CD73, also known as Ecto-5′-nucleotidase, catalyzes the conversion of purine 5′ mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation, being expressed on subsets of B-cells and T-cells. CD73 is also a marker of undifferentiated mesenchymal stem cells and is often used to identified stem cell populations.

Immunogen

Whole Mouse Dermal Lymphatic Endothelial Cells (DLEC)

Application

Evaluated by Immunocytochemistry on human bone marrow mesenchymal stem cells. Immunocytochemical analysis: A 1:50 dilutions of this antibody detected CD73 on 1X10^6 human adipose and bone marrow mesenchymal stem cells.
Research Category
Stem Cell Research
Research Sub Category
Mesenchymal Stem Cells
This Anti-CD73, clone AA60-E3-3 is validated for use in Immunocytochemistry for the detection of CD73.

Quality

Evaluated by Immunocytochemistry on human adipose mesenchymal stem cells.
Immunocytochemical analysis: A 1:50 dilutions of this antibody detected CD73 on 1X10^6 human adipose and bone marrow mesenchymal stem cells.

Target description

The unconjugated parent antibody (Catalog No. MABD122) has an observed MW at 63 kDa

Physical form

Protein G Purified
Purified mouse monoclonal IgG1κ conjugated to Alexa Fluor 488 in PBS with 0.1% sodium azide and 15mg/ml BSA.

Storage and Stability

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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