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MABE1006

Sigma-Aldrich

Anti-N6-methyladenosine Antibody (m6A), clone 17-3-4-1

clone 17-3-4-1, from mouse

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Synonym(s):
N6-methyladenosine, m6A
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

17-3-4-1, monoclonal

species reactivity (predicted by homology)

all

technique(s)

RIP: suitable
dot blot: suitable

isotype

IgG1λ

shipped in

wet ice

target post-translational modification

unmodified

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ZMS1124MABS1280MMABS1295
Anti-mS2i6A Antibody, clone CL-3 clone CL-3, from mouse

MABS1295

Anti-mS2i6A Antibody, clone CL-3

clone

17-3-4-1, monoclonal

clone

17-3-4-1, recombinant monoclonal

clone

CL-6, monoclonal

clone

CL-3, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

isotype

IgG1λ

isotype

IgG1λ

isotype

IgG2bκ

isotype

IgG2aκ

technique(s)

RIP: suitable, dot blot: suitable

technique(s)

dot blot: suitable, flow cytometry: suitable, immunocytochemistry: suitable

technique(s)

ELISA: suitable, dot blot: suitable

technique(s)

ELISA: suitable, dot blot: suitable, immunofluorescence: suitable, immunohistochemistry: suitable

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

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General description

N6-Methyladenosine (m6A ) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalyzed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment. Fat mass and obesity-associated (FTO) and ALKBH5 are two identified demethylases that catalyze oxidative demthylation of m6A. Several functions of m6A modification have been proposed, including mRNA decay, splicing regulation, RNA nuclear export and maturation. (PMID 25171402). Cat. No. MABE1006, Anti-N6-methyladenosine (m6A), clone 17-3-4-1, is a mouse monoclonal that specifically detects RNA molecules with m6A modication.

Immunogen

Epitope: N6-methyladenosine
Hapten N6-methyladenosine-5′-mono-phosphate conjugated to BSA of all N6-methyladenosine (m6A).

Application

(RNA) Immunoprecipitation Analysis: A representative lot immunoprecipitated N6-methyladenosine in IME2 transcripts (Bodi, Z., et al. (2010). Nucleic Acids Research. 38(16):5327-5335).
RNA Dot Blot Analysis: A representative lot detected N6-methyladenosine in m6A (Bodi, Z., et al. (2010). Nucleic Acids Research. 38(16):5327-5335).
Anti-N6-methyladenosine Antibody (m6A), clone 17-3-4-1 is an antibody against N6-methyladenosine for use in Dot Blot and RNA binding protein immunoprecipitation.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors

Quality

Evaluated by RNA Dot Blot in oligomers containing N6-methyladenosine.

RNA Dot Blot Analysis: 1.0-2.0 μg/mL of this antibody detected N6-methyladenosine in oligomers containing N6-methyladenosine.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1λ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Nate J Fry et al.
Oncotarget, 9(58), 31231-31243 (2018-08-23)
The mRNA modification N6-methyladenosine (m6A) is involved in many post-transcriptional regulatory processes including mRNA stability and translational efficiency. However, it is also imperative to correlate these processes with phenotypic outputs during cancer progression. Here we report that m6A levels are
Le Han et al.
Frontiers in molecular biosciences, 8, 780089-780089 (2022-02-04)
Insulin-like growth factor 2 (IGF2) mRNA-binding protein 2 (IGF2BP2) is an important posttranscriptional regulatory for stability and m6A modification. Here, we investigated the role of IGF2BP2 in non-small-cell lung cancer (NSCLC) proliferation. TCGA database was used to predict the expression
Rong-Zhang He et al.
Cancer cell international, 21(1), 616-616 (2021-11-24)
UCA1 is frequently upregulated in a variety of cancers, including CRC, and it can play an oncogenic role by various mechanisms. However, how UCA1 is regulated in cancer is largely unknown. In this study, we aimed to determine whether RNA
Kang Wei et al.
Bioengineered, 13(3), 5236-5250 (2022-02-15)
N6-methyladenosine (m6A) is one of the most significant modifications in human mRNAs. Emerging evidence indicates that m6A participates in the initiation and development of malignant tumors. Nevertheless, the biological roles and mechanism of m6A in osteosarcoma (OS) remain unclear. The
Junfei Han et al.
Frontiers in cell and developmental biology, 9, 659228-659228 (2022-02-11)
Glioma is the most common type of malignant tumor of the nervous system and is characterized by high mortality and poor outcome. This study aims to investigate the mechanism underlying IGF2 mRNA-binding protein 2 (IGF2BP2) and long noncoding RNA DANCR

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