MABE656
Anti-HP1γ Antibody, clone 14D3.1
clone 14D3.1, from mouse
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Synonym(s):
Chromobox protein homolog 3, HECH, Heterochromatin protein 1 homolog gamma, HP1 gamma, Modifier 2 protein
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
14D3.1, monoclonal
species reactivity
human
species reactivity (predicted by homology)
rat (based on 100% sequence homology), mouse (based on 100% sequence homology), nonhuman primates (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... CBX3(11335)
General description
Chromobox protein homolog 3 (UniProt Q13185; also known as HECH, Heterochromatin protein 1 homolog gamma, HP1 gamma, Modifier 2 protein) is encoded by the CBX3 gene (Gene ID 11335) in human. HP1 is a non-histone chromosomal protein that is present as three orthologs, HP1-alpha, HP1-beta, and HP1-gamma, in mammals. While HP1-alpha and HP1-beta have been unambiguously localized in heterochromatin, HP1-gamma has been found to localize in both euchromatin and heterochromatin (transiently) compartments of interphase nuclei, as well as in the pericentromeric chromatin and arms of mitotic chromosomes of 3T3 cells. HP1-gamma is phosphorylated by PIM1 during interphase and may be hyper-phosphorylated during mitosis. HPI-gamma is involved in transcriptional silencing in heterochromatin-like complexes. It recognizes and binds histone H3 tails methylated at ′Lys-9′, leading to epigenetic repression. It may also contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). It is also reported to be involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Ref.: Minc E., et al. (2000). Cytogenet. Cell Genet. 90, 279-284.
Lachner, M., et al. (2001). Nature 410, 116-120.
Lachner, M., et al. (2001). Nature 410, 116-120.
Immunogen
KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human HP1γ.
Application
Anti-HP1γ, clone 14D3.1, Cat. No. MABE656, is a highly specific mouse monoclonal antibody that targets heterochromatin protein-1 gamma and has been tested in Immunocytochemistry and Western Blotting.
Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected nuclear HP1γ immunoreactivity in 4% paraformaldehyde-fixed, 0.1 Triton X-100-permeabilized A431 and HeLa cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Quality
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected HP1γ in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected HP1γ in 10 µg of HeLa cell lysate.
Target description
~20 kDa observed. 20.68 kDa(Met1 removed) calculated. Uncharacterized bands may be observed in some lysate(s).
Linkage
Replaces: MAB3450
Physical form
Format: Purified
Protein G purified.
Purified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
wgk_germany
WGK 1
Certificates of Analysis (COA)
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Zhaohua Hu et al.
The EMBO journal, 39(12), e104133-e104133 (2020-04-30)
Long non-coding RNAs (lncRNAs) are emerging regulators of genomic stability and human disease. However, the molecular mechanisms by which nuclear lncRNAs directly contribute to DNA damage responses remain largely unknown. Using RNA antisense purification coupled with quantitative mass spectrometry (RAP-qMS)
Kosuke Sako et al.
The Journal of cell biology, 223(9) (2024-05-23)
Maintenance of ploidy depends on the mitotic kinase Aurora B, the catalytic subunit of the chromosomal passenger complex (CPC) whose proficient activity is supported by HP1 enriched at inner centromeres. HP1 is known to associate with INCENP of the CPC
Yihan Peng et al.
Nature communications, 10(1), 1224-1224 (2019-03-16)
Poly-(ADP-ribose) polymerase inhibitors (PARPi) selectively kill breast and ovarian cancers with defects in homologous recombination (HR) caused by BRCA1/2 mutations. There is also clinical evidence for the utility of PARPi in breast and ovarian cancers without BRCA mutations, but the
Ali Fathi et al.
Aging cell, 21(1), e13541-e13541 (2021-12-26)
Modeling age-related neurodegenerative disorders with human stem cells are difficult due to the embryonic nature of stem cell-derived neurons. We developed a chemical cocktail to induce senescence of iPSC-derived neurons to address this challenge. We first screened small molecules that
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