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MABF163D

Sigma-Aldrich

Anti-CD20 (human), violetFluor® 450, clone 2H7 Antibody

clone 2H7, from mouse, violetFluor® 450

Synonym(s):

B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD antigen CD20, CD20

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43

biological source

mouse

Quality Level

100

conjugate

violetFluor® 450

antibody form

purified antibody

antibody product type

primary antibodies

clone

2H7, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable

UniProt accession no.

P11836

target post-translational modification

unmodified

Gene Information

human ... MS4A1(931)

General description

The 2H7 antibody reacts with human CD20, expressed by pre-B cells, resting and activated B cells, follicular dendritic cells and a small subset of T cells, but not on plasma cells. CD20 is a 33-37 kDa transmembrane protein which is differentially phosphorylated in resting and active B cells. The CD20 molecule plays a role in regulating B-cell activation.The 2H7 antibody is reported to be cross-reactive with several non-human species including Baboon, Chimpanzee, Cynomolgus and Rhesus.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This mouse monoclonal Anti-CD20 Antibody (human), violetFluor® 450, clone 2H7, Cat. No. MABF1639 is validated for use in Flow Cytometry, for the detection of CD20.

Quality

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 μg from a representative lot detected CD20 in one million human peripheral blood lymphocytes.

Physical form

Purified Mouse monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Purified by size-exclusion chromatography

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

violetFluor is a registered trademark of Tonbo Biotechnologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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