MABF290

Anti-S100A9 Antibody, clone 2A5 (Azide Free)

clone 2A5, 1 mg/mL, from rat

• Synonym(s): Protein S100-A9, Calgranulin-B, Leukocyte L1 complex heavy chain, Migration inhibitory factor-related protein 14, MRP-14, p14, S100 calcium-binding protein A9
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2A5, monoclonal
• species reactivity: human, mouse
• concentration: 1 mg/mL
• technique(s): activity assay: suitable; western blot: suitable
• isotype: IgG1
• NCBI accession no.: NP_00126878
• UniProt accession no.: P31725
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... S100A9(6280)

Description

General description

S100A9, also known as Calgranulin-B, Leukocyte L1 complex heavy chain, Migration inhibitory factor-related protein 14, MRP-14, p14, or S100 calcium-binding protein A9, and encoded by the gene S100A9/CAGB/MRP14/S100-A9 is a calcium and zinc binding protein that plays a prominent role in the regulation of inflammatory processes and immune responses. S100-A9 can induce neutrophil chemotaxis and adhesion. S100-A9 one of the subunits of Calprotectin, which is a complex of S100-A8 and S100-A9 proteins and is also critical for immune responses. S100-A9 is a cytoplasmic and secreted protein predominantly expressed in myeloid cells as well as neutrophils and monocytes but not expressed in lymphocytes. S100-A9 is found in high concentrations at sites of inflammation or in the serum of patients with inflammatory diseases. S100-A9 is also a cancer marker as it is up regulated in many cancers including gastric, esophageal, colon, breast and many more.

Immunogen

Recombinant protein corresponding to mouse S100A9.

Application

Activity Assay Analysis: A representative lot detected S100A9 and found that it increases neutrophil migration & induces the secretion of cytokines by monocytes (Cesaro, A., et al. (2012). PLOS ONE. 7(9):e45478 1-12).
Western Blotting Analysis: A representative lot detected S100A9 in mouse neutrophils and recombinant mS100A9 (Cesaro, A., et al. (2012). PLOS ONE. 7(9):e45478 1-12).

Anti-S100A9 Antibody, clone 2A5 (Azide Free) is an antibody against S100A9 for use in western blotting & activity assay.

Quality

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected S100A9 in 10 µg of Raji cell lysate.

Target description

~15 kDa observed

Physical form

Format: Purified

Purified rat monoclonal IgG1 in buffer containing PBS without preservatives.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable