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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
3A7.10, monoclonal
mol wt
calculated mol wt 23.72 kDa
observed mol wt ~21 kDa
purified by
using protein G
species reactivity
human
packaging
antibody small pack of 100 μg
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG1κ
epitope sequence
Unknown
Protein ID accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
mouse ... IL6(3569)
General description
Interleukin-6 (UniProt: P05231; also known as IL-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor, Interferon beta-2, IFN-beta-2) is encoded by the IL6 (also known as IFNB2) gene (Gene ID: 3569) in human. IL-6 is an immunoregulatory secreted cytokine that is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off to produce the mature form (aa 30-212). It is mainly produced in skeletal muscle and its levels are shown to increase upon exercise due to enhanced production by contracting skeletal muscles. It is a potent inducer of the acute phase response and rapid IL-6 production contributes to host defense during infection and tissue injury. In the innate immune response, it is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection. In the adaptive immune response, it is required for the differentiation of B cells into immunoglobulin-secreting cells. It is reported to plays a major role in the differentiation of CD4+ T cell subsets and is an essential factor for the development of T follicular helper (Tfh) cells. Its actions are mediated by binding to IL-6 receptor (IL-6R) and the ligand-receptor complex associates to the signaling subunit IL6ST/gp130. The interaction with the membrane-bound IL6R and IL6ST is reported to stimulate the classic signaling, whereas the binding of IL6 and soluble IL6R to IL6ST stimulates trans-signaling where synergistically with IL-1b and TNF it can induce the production of VEGF and stimulate angiogenesis. (Ref.: Nakahara, H., et al. (2003). Arthritis Rheum. 48(6); 1521-1529; Boulanger, MJ., et al. (2003). Science. 300(5628); 2101-2104).
Specificity
Clone 3A7.10 is a mouse monoclonal antibody that detects human IL-6.
Immunogen
Recombinant fragment of human Interleukin 6 (IL-6) corresponding to amino acids 29-212.
Application
Quality Control Testing
Evaluated by Western Blotting with recombinant human IL-6.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant human IL-6.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-6 in human tonsil tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting with recombinant human IL-6.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant human IL-6.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-6 in human tonsil tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-IL-6, clone 3A7.10, Cat. No. MABF3179, is a mouse monoclonal antibody that detects human Interleukin-6 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Physical form
Purified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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