MABF3371

Anti-C5aR1/CD88 Antibody, clone P12/1

• Synonym(s): C5a anaphylatoxin chemotactic receptor, C5a anaphylatoxin chemotactic receptor 1, C5a-R, CD88
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: P12/1, monoclonal
• mol wt: calculated mol wt 39.34 kDa; observed mol wt ~N/A kDa
• purified by: using protein G
• species reactivity: human
• packaging: antibody small pack of 100
• technique(s): electron microscopy: suitable; flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG2aκ
• epitope sequence: N-terminal extracellular domain
• Protein ID accession no.: NP_001727
• UniProt accession no.: P21730
• storage temp.: 2-8°C
• Gene Information: human ... C5AR1(728)

Description

Specificity

Clone P12/1 is a mouse monoclonal antibody that detects C5aR1/CD88. It targets an epitope within the first extracellular domain from the N-terminal region.

Immunogen

BSA-conjugated linear peptide corresponding to 31 amino acids within the first extracellular domain from the N-terminal region of human C5aR1.

Application

Quality Control Testing

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis: 1.0 µg of this antibody detected C5aR1/CD88 in one million Human peripheral blood mononuclear cells (PBMC).

Tested Applications

Flow Cytometry Analysis: A representative lot detected C5aR1/CD88 in Flow Cytometry application. (Werfel, T., et al. (1996). J Immunol. 157(4):1729-35).

Western Blotting Analysis: A representative lot detected C5aR1/CD88 in Western Blotting application. (Huttenrauch, F., et al. (2005). J Biol Chem. 280(45): 37503-15).

Electron Microscopy: A representative lot detected C5aR1/CD88 in Electron Microscopy application. (Werfel, T., et al. (1996). J Immunol. 157(4): 1729-35).

Immunohistochemistry Applications: A representative lot detected C5aR1/CD88 in Immunohistochemistry application. (Werfel, T., et al. (1996). J Immunol. 157(4): 1729-35).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

C5a anaphylatoxin chemotactic receptor 1 (UniProt: P21730; also known as C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, CD88) is encoded by the C5AR1 (also known as C5AR, C5R1) gene (Gene ID: 728) in human. The C5a anaphylatoxin is a potent complement-derived mediator of inflammation whose actions are mediated via the C5a chemotactic receptor (C5aR). C5aR is a multi-pass membrane protein that serves as a receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. It exists as a homodimer but can also form higher-order oligomers. C5a interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region that activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, and intracellular calcium release and superoxide anion production. Chemotaxis inhibitory protein of Staphylococcus aureus (CHIPS), a 121-residue protein, is reported to bind to the N-terminus of the C5aR (aa 1-35) with nanomolar affinity and inhibit C5a-mediated responses in human leukocytes. Sulfation at Tyrosine 14 is shown to be important for CHIPS binding. Upon C5a binding, C5aR undergoes rapid phosphorylation on the six serine residues present in the C-terminal region followed by desensitization and internalization. This agonist-induced phosphorylation is a critical for the functional alteration of the receptor during desensitization. The key residues involved in this process are Serine 334 and Serine 338. Phosphorylated C5aR is shown to co-localize with beta-arrestin 1 and 2 in cytoplasmic vesicles. Although C5aR activation is able to promote a loose association with β-arrestins, but phosphorylation of either Serine 334/338 or Serine 327)/338 is reported to be essential for the formation of a persistent complex. (Ref.: Braun, L., et al. (2003). J. Biol. Chem. 278(6); 4277-4285; Oppermann, M., et al. (1994). Immunology. 82(4); 516-521).

Physical form

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable