MABF471

Anti-Ly-6G Antibody (mouse), PerCP-Cy5.5, clone 1A8

clone 1A8, 0.2 mg/mL, from rat

• Synonym(s): Lymphocyte antigen 6G, Ly-6G, Ly-6G.1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• conjugate: PerCP-Cy5.5
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1A8, monoclonal
• species reactivity: mouse
• concentration: 0.2 mg/mL
• technique(s): flow cytometry: suitable
• isotype: IgG2aκ
• UniProt accession no.: P35461
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Ly6G(546644)

Description

General description

The 1A8 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the 1A8 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes. Note: for identification of Ly-C, an alternative antibody, clone RB6-8C5, has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C.

This rat monoclonal antibody Anti-Ly-6G (mouse), PerCP-Cy5.5 & clone 1A8 validated for use in Flow Cytometry for the detection of Ly-6G.

Quality

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 µg of this antibody detected Ly-6G in one million C57Bl/6 bone marrow cells.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable