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MABF550

Sigma-Aldrich

Anti-CD25 Antibody (mouse), PerCP-Cy5.5, clone PC61.5

clone PC61.5, 0.2 mg/mL, from rat

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Synonym(s):
Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, p55, CD25
eCl@ss:
32160702
NACRES:
NA.44

biological source

rat

Quality Level

conjugate

PerCP-Cy5.5

antibody form

purified antibody

antibody product type

primary antibodies

clone

PC61.5, monoclonal

species reactivity

mouse

packaging

antibody small pack of 25 μg

concentration

0.2 mg/mL

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG1λ

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Il2Ra(16184)

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MABF397MABF549MABF548
antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

Gene Information

mouse ... Il2Ra(16184)

Gene Information

mouse ... Il2Ra(16184)

Gene Information

mouse ... Il2Ra(16184)

Gene Information

mouse ... Il2Ra(16184)

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

clone

PC61.5, monoclonal

clone

PC61.5, monoclonal

clone

PC61.5, monoclonal

clone

PC61.5, monoclonal

biological source

rat

biological source

rat

biological source

rat

biological source

rat

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General description

The PC61.5 antibody is specific for mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, althoµgh with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Expression of CD25 varies during developmental stages of T and B cells, is induced on activated mature T and B cells, and is present on subsets of dendritic cells. CD25 signaling as part of the IL-2 receptor complex triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells.
The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used for depletion of Treg cells in vivo, as well as to distinguish Treg cells from naïve or conventional T cells which are CD25-.

Application

This rat monoclonal antibody Anti-CD25 (mouse), PerCP-Cy5.5, clone PC61.5 validated for use in IP, IHC & Flow Cytometry for the detection of CD25.

Quality

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 µg of this antibody detected CD25 in one million C57Bl/6 splenocytes.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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