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MABN103

Sigma-Aldrich

Anti-GLUR2 pS880 (Clone 022.19.5) Antibody

clone 022.19.5, from mouse

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Synonym(s):
Glutamate receptor 2, GluR-2, AMPA-selective glutamate receptor 2, GluR-B, GluR-K2, Glutamate receptor ionotropic, AMPA 2, GluA2
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

022.19.5, monoclonal

species reactivity

rat

technique(s)

western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

phosphorylation (pSer880)

Gene Information

Rattus norvegicus ... Gria2(29627)

General description

Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. Although subunit composition of AMPA receptors varies, they must contain at least one edited GluR2 subunit to be calcium impermeable. Ser880 has been identified as the protein kinase C (PKC) phosphorylation site within the C-terminal region of GluR2 and has been shown to differentially regulate the interaction of the PDZ domain-containing proteins GRIP1 and PICK 1. In addition, PKC phosphorylation of GluR2 Ser880 appears to be a critical event in the induction of cerebellar long term depression (LTD).

Specificity

This antibody recognizes GluR2 phosphorylated at Ser880.

Immunogen

Epitope: Phosphorylated Ser880
KLH-conjugated linear peptide corresponding to rat GluR2 phosphorylated at Ser880.

Application

Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Neurodegenerative Diseases
This Anti-GLUR2 pS880 (Clone 022.19.5) Antibody is validated for use in WB for the detection of GLUR2 pS880 (Clone 022.19.5).

Quality

Evaluated by Western Blot in lambda phosphatase treated and untreated rat hippocampus tissue lysates.

Western Blot Analysis: 1 µg/mL of this antibody detected GluR2 on 10 µg of lambda phosphatase untreated rat hippocampus tissue lysate.

Target description

~100 kDa observed

Physical form

Format: Purified
Protein G
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Lambda phosphatase treated and untreated rat hippocampus tissue lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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