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MABN2322

Sigma-Aldrich

Anti-Nerve Growth Factor Antibody, clone α D11

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Synonym(s):
NGF;beta-NGF

biological source

rat

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

α D11, monoclonal

mol wt

calculated mol wt 27.088 kDa

purified by

using protein G

species reactivity

chicken, human, mouse

packaging

antibody small pack of 100 μg

technique(s)

ELISA: suitable

isotype

IgG2a

epitope sequence

N-terminal half

Protein ID accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

mouse ... Ngf(18049)

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This Item
N6655N3279N3908
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

species reactivity

chicken, human, mouse

species reactivity

mouse, human

species reactivity

human, rat, mouse

species reactivity

rat

clone

α D11, monoclonal

clone

polyclonal

clone

25623, monoclonal

clone

polyclonal

Gene Information

mouse ... Ngf(18049)

Gene Information

human ... NGF(4803)
mouse ... Ngf(18049)

Gene Information

human ... NGF(4803)
mouse ... Ngf(18049)

Gene Information

rat ... Ngfr(24596)

antibody form

purified antibody

antibody form

fractionated antiserum

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

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General description

Beta-nerve growth factor (UniProt: P01139; also known as Beta-NGF) is encoded by the Ngf (also known as Ngfb) gene (Gene ID: 18049) in murine species. β-NGF, an endogenous neurotrophic factor, is a homodimeric glycoprotein that is synthesized with a signal peptide (aa 1-18) and a propeptide (aa 19-121) that are subsequently cleaved off to generate the mature form (aa 122-241). It is highly expressed in male submaxillary gland and its levels are much lower in female submaxillary gland. Its expression is reported to oscillate in a circadian manner in the suprachiasmatic nucleus (SCN) of the brain. NGF is important for the development and maintenance of the sympathetic and sensory nervous systems. It serves as an extracellular ligand for the NTRK1 and NGF receptors to activate cellular signaling cascades to regulate neuronal proliferation, differentiation, and survival. However, it does not permeate the blood-brain barrier. Its precursor form (ProNGF) is reported to function as a ligand for the heterodimeric receptor formed by Sortilin Related VPS10 Domain Containing Receptor 2 (SORCS2) and NGF receptor that activates signaling cascade leading to inactivation of RAC1 and/or RAC2. In contrast to the mature form, ProNGF is known to promote neuronal apoptosis. N-glycosylation of ProNGF is reported to be essential for its processing, secretion, and construction of the tertiary fold of the β-NGF homodimer. Clone D11 is a potent antagonist that can effectively antagonize rodent and human NGF in a variety of in vitro and in vivo systems. (Ref.: Covaceuszach, S., et al. (2012). PLoS ONE 7(3): e32212; Feng, D., et al. (2010). J. Mol. Biol. 396(4); 967-984; Scott, J., et al. (1983). Nature. 302(5908); 538-540).

Specificity

Clone D11 is a rat monoclonal antibody that detects Nerve growth factor (NGF). It targets an epitope within 23 amino acids from the N-terminal half of the mature NGF.

Immunogen

2.5S NGF purified from the mouse submandibular glands.

Application

Quality Control Testing

Evaluated by ELISA with recombinant mouse Nerve growth factor.

ELISA Analysis (ELISA): A representative lot of this antibody detected various dilution of recombinant mouse Nerve growth factor.

Tested applications

Surface plasmon resonance: A representative lot detected Nerve Growth Factor in Surface plasmon resonance applications (Covaceuszach, S., et al. (2012). PLoS One. 7(3):e32212).

ELISA Analysis: A representative lot detected Nerve Growth Factor in ELISA applications (Cattaneo, A., et al. (1988). J. Neurochem. 50(4):1003-10; Covaceuszach, S., et al. (2012). PLoS One. 7(3):e32212).

Inhibition Assay: A representative lot competitively inhibited the biological activity of mNGF and prevented the binding of NGF to PC12 cells. (Cattaneo, A., et al. (1988). J. Neurochem. 50(4):1003-10).

Radioimmunoassay: A representative lot detected Nerve growth factor in Radioimmunoassay applications (Cattaneo, A., et al. (1988). J. Neurochem. 50(4):1003-10).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Nerve Growth Factor, clone D11, Cat. No. MABN2322, is a rat monoclonal antibody that detects Nerve growth factor and is tested for use in ELISA, Inhibition Assay, Radioimmunoassay, and Surface Plasmon Resonance.

Physical form

Purified rat monoclonal antibody IgG2a in PBS without preservatives.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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