MABN2724

Anti-Sonic Hedgehog/Shh Antibody, clone 5E1

• Synonym(s): EC:3.1., SHH, Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains, ShhNC
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 5E1, monoclonal
• mol wt: calculated mol wt 47.63 kDa; observed mol wt ~47 kDa
• purified by: using protein G
• species reactivity: mouse, chicken, human, rat
• packaging: antibody small pack of 100
• technique(s): electron microscopy: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; inhibition assay: suitable (peptide); western blot: suitable
• isotype: IgG1κ
• epitope sequence: N-terminal half
• Protein ID accession no.: NP_058917
• UniProt accession no.: Q63673
• storage temp.: -10 to -25°C
• Gene Information: rat ... Shh(29499)

Description

Specificity

Clone 5E1 is a mouse monoclonal antibody that specifically detects Sonic Hedgehog protein. It targets an epitope within 22 amino acids from the N-terminal half.

Immunogen

N-terminal, recombinant fragment of rat Sonic Hedgehog protein.

Application

Quality Control Testing

Evaluated by Western Blotting in rat entorhinal cortex (EC) tissue lysate.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected Sonic Hedgehog in rat entorhinal cortex (EC) tissue lysate.

Tested Applications

Inhibition Assay: A representative lot inhibited Sonic hedgehog signaling (Maun, H.R., et al. (2010). 285(34):26570-80; Voronova, A., et al. (2013). J Biol Chem. 288(6):4389-404; Lusche, D.F., et al. (2019). MAbs. 11(4):691-708).

Immunocytochemistry Analysis: A representative lot detected Sonic hedgehog in Immunocytochemistry applications (Eitan, E., et al. (2016). Biol Open. 5(8):1086-92).

Immunofluorescence Analysis: A representative lot detected Sonic hedgehog in Immunofluorescence applications (Lewis, W.R., et al. (2016). PLoS Genet. 12(7):e1006220; Orquera, D.P., et al. (2016). Dev Biol. 416(1):212-224).

Immunohistochemistry Applications: A representative lot detected Sonic Hedgehog/Shh in Immunohistochemistry applications (Reeber, S.L., et al. (2008). J Neurosci. 28(35):8698-708; Maun, H.R., et al. (2010). 285(34):26570-80; Orquera, D.P., et al. (2016). Dev Biol. 416(1):212-224).

Western Blotting Analysis: A representative lot detected Sonic Hedgehog/Shh in Western Blotting applications (Coffman, L.G., et al. (2016). Oncotarget. 7(6):6916-32; Eitan, E., et al. (2016). Biol Open. 5(8):1086-92).

Electron Microscopy: A representative lot detected Sonic Hedgehog/Shh in Electron Microscopy applications (Eitan, E., et al. (2016). Biol Open. 5(8):1086-92).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Sonic hedgehog protein (UniProt: Q63673; also known as SHH, EC:3.1.-.-, ShhNC, ShhN) is encoded by the Shh (also known as Vhh-1 ) gene (Gene ID: 29499) in rat. The Sonic Hedgehog (Shh) protein is a member of the hedgehog family of signaling molecules which also include Indian Hh and Desert Hh. It is initially synthesized as a precursor protein. The C-terminal domain of the precursor SHH displays an autoproteolysis activity and a cholesterol transferase activity, causing a cleavage of the precursor SHH into an N-terminal fragment (ShhN-product; aa 25-198) as well as the covalent attachment of a cholesterol moiety to the C-terminal end of the N-product. The N-product is the active signaling species, whereas the C-product has no signaling activity. Shh expression is highly regulated and varies depending on the developmental stage and tissue type. In the developing embryo, Shh is expressed in the notochord, floor plate of the neural tube, limb buds, and various other tissues. In adults, Shh expression is more restricted but remains important for tissue homeostasis and repair. The precursor protein is processed in the endoplasmic reticulum and Golgi apparatus before being transported to the cell membrane. The dual-lipidated sonic hedgehog protein N-product (ShhNp) is firmly tethered to the cell membrane where it forms multimers. Dysregulation of Shh signaling can lead to various congenital disorders and diseases. The intercellular signaling triggered by SHH is essential for a variety of patterning events during development including the development of the notochord, neural tube and plate, as well as the polarizing of the anterior-posterior axis of developing limb buds. Because of its importance in patterning during development, defects in SHH are the cause of a number of abnormalities including structural eye and brain abnormalities, teeth and bone malformations and finally limb and bone developmental defects. (Ref.: Reeber, S.L., et al. (2008). J Neurosci. 28(35):8698-708; Maun, H.R., et al. (2010). 285(34):26570-80; Voronova, A., et al. (2013). J Biol Chem. 288(6):4389-404; Coffman, L.G., et al. (2016). Oncotarget. 7(6):6916-32; Eitan, E., et al. (2016). Biol Open. 5(8):1086-92; Lewis, W.R., et al. (2016). PLoS Genet. 12(7):e1006220; Orquera, D.P., et al. (2016). Dev Biol. 416(1):212-224; Lusche, D.F., et al. (2019). MAbs. 11(4):691-708.)

Physical form

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable