MABN486
Anti-Co-Rest/RCOR1 Antibody, clone K72/8
clone K72/8, from mouse
Synonym(s):
REST corepressor 1, Protein CoREST
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
K72/8, monoclonal
species reactivity
rat, human, mouse
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... RCOR1(23186)
General description
REST corepressor 1(RCOR1, CoREST) is an essential component of the BHC corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. RCOR1 also promotes the demethylase activity of KDM1A on core histones and nucleosomal substrates and plays a central role in demethylation of Lys-4 of histone H3. Furthermore, RCOR1 is a component of a RCOR/GFI/KDM1A/HDAC complex that suppresses a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. RCOR1 is ubiquitously expressed and is primarily found in the nucleus, but is partially translocated into the cytoplasm upon HSV-1 infection.
Specificity
This antibody may cross react with human RCOR3 based upon 70% sequence homology.
Immunogen
Recombinant protein corresponding to human Co-Rest/RCOR1.
Application
Anti-Co-Rest/RCOR1 Antibody, clone K72/8 is a highly specific mouse monoclonal antibody, that targets CoRest & has been tested in western blotting, IHC & ICC.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Co-Rest/RCOR1 in human stomach tissue.
Immunocytochemistry Analysis: A representative lot detected Co-Rest/RCOR1 in COS-1 cells expressing Myc-tagged Co-Rest. (Prof. J. Trimmer, University of California, Davis.).
Immunocytochemistry Analysis: A representative lot detected Co-Rest/RCOR1 in COS-1 cells expressing Myc-tagged Co-Rest. (Prof. J. Trimmer, University of California, Davis.).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Signaling
Developmental Signaling
Quality
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Co-Rest/RCOR1 in 10 µg of Jurkat cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Co-Rest/RCOR1 in 10 µg of Jurkat cell lysate.
Target description
~ 65 kDa observed. The calculated molecular weight is 53 kDa, however Co-Rest/RCOR1 has been shown as a ~65-70 kDa band in western blots (You, A., et al. (2001). National Academy of Sciences. 98(4):1454-1458)(Min, G. L., et al. (2005). Nature. 437:432-435).
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Jurkat cell lysate
Jurkat cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Yan Xiong et al.
The Journal of clinical investigation, 130(4), 1830-1842 (2020-01-10)
Foxp3+ Tregs are key to immune homeostasis, but the contributions of various large, multiprotein complexes that regulate gene expression remain unexplored. We analyzed the role in Tregs of the evolutionarily conserved CoREST complex, consisting of a scaffolding protein, Rcor1 or
Masaki Hiramoto et al.
International journal of molecular medicine, 41(2), 717-728 (2017-12-06)
Although genome-wide association studies have shown that potassium voltage-gated channel subfamily Q member 1 (KCNQ1) is one of the genes that is most significantly associated with type 2 diabetes mellitus (T2DM), functionally annotating disease-associated single nucleotide polymorphisms (SNPs) remains a challenge. Recently, our group described
Seda Kilinc et al.
Molecular and cellular neurosciences, 74, 58-70 (2016-03-08)
Mammalian olfaction depends on the development of specialized olfactory sensory neurons (OSNs) that each express one odorant receptor (OR) protein from a large family of OR genes encoded in the genome. The lysine-specific demethylase-1 (LSD1) protein removes activating H3K4 or
Rutesh N Vyas et al.
Molecular and cellular neurosciences, 82, 1-11 (2017-04-18)
Function of the mammalian olfactory system depends on specialized olfactory sensory neurons (OSNs) that each express only one allele ("monoallelic") of one odorant receptor (OR) gene ("monogenic"). The lysine-specific demethylase-1 (LSD1) protein removes activating H3K4 or silencing H3K9 methylation marks
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