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Key Documents

MABN621

Sigma-Aldrich

Anti-Pan-Neurofascin Antibody, clone L11A/41

clone L11A/41, from mouse

Synonym(s):

Neurofascin

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

L11A/41, monoclonal

species reactivity

rat

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

General description

Neurofascin is a ankyrin binding protein that may be involved in myelination, neurite extension, synaptogenesis, axonal guidance and neuron-glial cell interactions. There are two known isoforms, NF155 and NF186. NF155 is found in oligodendrocytes, and is involved in neurite outgrowth and neuronal adhesion. NF186 is expressed in neurons, and may optimize communication between neurons by anchoring the axon initial segment (AIS).

Specificity

Other Homologies: Human (99% sequence homology).

Immunogen

Recombinant protein corresponding to rat Pan-Neurofascin.

Application

Detect Neurofascin using this mouse monoclonal antibody, Anti-Pan-Neurofascin Antibody, clone L11A/41 validated for use in western blotting & IHC.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Pan-Neuorfascin in rat pons tissue.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Pan-Neurofascin in 10 µg of rat brain tissue lysate.

Target description

~185 kDa and ~155 kDa observed. Three isoforms are known to exist at 138 kDa, 134 kDa, and 132 kDa due to alternative splicing. This protein has been observed at ~186 kDa, ~180 kDa, ~166 kDa and ~155 kDa (Kriebel, M., et al. (2012). Int J Biochem Cell Biol. 44(5):694-697.). This protein may undergo post-translational modification.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nadav I Weinstock et al.
Neuron, 107(1), 65-81 (2020-05-07)
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