MABN721

Anti-PTK7 Antibody, clone 4F9

ascites fluid, clone 4F9, from mouse

• Synonym(s): Inactive tyrosine-protein kinase 7, CCK-4, Protein-tyrosine kinase 7, Pseudo tyrosine kinase receptor 7, Tyrosine-protein kinase-like 7
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 4F9, monoclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1
• NCBI accession no.: NP_002812
• UniProt accession no.: Q13308
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PTK7(5754)

Description

General description

The gene PTK7 encodes the protein named Inactive tyrosine-protein kinase 7 or Protein-tyrosine kinase 7, or colon carcinoma kinase 4 (CCK4). The PTK7 encoded protein is an interesting single pass type 1 membrane protein involved in Wnt signaling. It is interesting because it contains an inactive tyrosine kinase domain yet it is a component of both the canonical Wnt and non-canonical signaling pathways, and operates during embryogenesis and angiogenesis. Moreover, the PTK7 protein functions in cell migration, cell adhesion, cell polarity, cytoskeleton reorganization and apoptosis, and the PTK7 encoded protein co-localizes with MMP14 at cell junctions as well as the leading edge of migrating cells. The PTK7 encoded protein is expressed in lung, liver, pancreas and other tissues like kidney, placenta and melanocytes. The PTK7 encoded protein does not seem to be expressed in colon and has low expression in brain, heart, and skeletal muscle. EMD-Millipore’s Anti-PTK7 mouse monoclonal, clone 4F9 has been tested by western blot against multiple human cancer cells lines and in immunohistochemistry using paraffin-embedded human lung cancer tissue with DAB detection.

Immunogen

Purified protein fragment of human PTK7 expressed in E. coli.

Application

Detect Protein-tyrosine kinase 7 using this mouse monoclonal antibody, Anti-PTK7 Antibody, clone 4F9 validated for use in western blotting & IHC.

Immunohistochemistry Analysis: A 1:100 and 1:500 dilution from a representative lot detected PTK7 in human lung and kidney tissues.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis:A 1:1,000 dilution of this antibody detected PTK7 in 10 µg of A431 cell lysate.

Target description

~130 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 6 isoforms between ~90 kDa and ~119 kDa

Physical form

Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
A431 cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• wgk_germany: nwg