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MABN92-AF555

Sigma-Aldrich

Anti-Iba1/AIF1 Antibody, clone 20A12.1, Alexa Fluor 555 Conjugate

clone 20A12.1, from mouse, ALEXA FLUOR 555

Synonym(s):

Allograft inflammatory factor 1, AIF-1, Daintain, Interferon gamma responsive transcript, Ionized calcium-binding adapter molecule 1, Protein G1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.45

biological source

mouse

Quality Level

conjugate

ALEXA FLUOR 555

antibody form

purified antibody

antibody product type

primary antibodies

clone

20A12.1, monoclonal

species reactivity

rat, mouse, human

technique(s)

immunohistochemistry: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... AIF1(199)

General description

Allograft inflammatory factor 1 (UniProt P55008; also known as AIF-1, Daintain, Interferon gamma responsive transcript, Ionized calcium-binding adapter molecule 1, Protein G1) is encoded by the AIF1 (also known as G1, IBA1) gene (Gene ID 199) in human. Iba1/AIF1 is a 17 kDa interferon-gamma- (IFN-gamma-) inducible, Ca(2+)-binding EF-hand protein. Iba1/AIF1 expression is mostly limited to the macrophage/monocyte lineage, and is augmented by cytokines, such as IFN-gamma. It is reported to be involved in neuronal function, inflammatory responses, allograft rejection, autoimmunity, and macrophage activation/function. It is also shown to be expressed in microglial cells in autominnume disesase models such as experimental autoimmune encephalomyelitis, neuritis and uveitis.

Immunogen

Linear peptide corresponding to a human Iba1/AIF1 sequence.

Application

Anti-Iba1/AIF1 Antibody, clone 20A12.1, Alexa Fluor 555 Conjugate is an antibody against Iba1/AIF1 for use in Immunohistochemistry.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
The unconjugated antibody (Cat. No. MABN92) is shown to be suitable also for Western blotting and immunohistochemistry applications.

Quality

Evaluated by Immunohistochemistry in human spleen tissue.

Immunohistochemistry Analysis: A 1:150 dilution of this antibody detected Iba1/AIF1 in human spleen tissue.

Target description

~17 kDa observed

Physical form

Protein G Purified
Purified mouse antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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