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MABS1251

Sigma-Aldrich

Anti-CYP11B2 Antibody, clone 41-17B

clone 41-17B, from mouse

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Synonym(s):
Cytochrome P450 11B2, mitochondrial, CYP11B2, Aldosterone synthase, ALDOS, Aldosterone-synthesizing enzyme, CYPXIB2, Cytochrome P-450Aldo, Cytochrome P-450C18, Steroid 18-hydroxylase
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

41-17B, monoclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology)

technique(s)

immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CYP11B2(1585)

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MABS502MABS497MAB6021
antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

culture supernatant

clone

41-17B, monoclonal

clone

80-7, monoclonal

clone

4B7.2, monoclonal

clone

1G5, monoclonal

species reactivity

human

species reactivity

human, rat

species reactivity

mouse, rat, human

species reactivity

rat, mouse

technique(s)

immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

isotype

IgG1κ

isotype

IgG2aκ

isotype

IgG2aκ

isotype

IgG1κ

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General description

Cytochrome P450 11B2, mitochondrial (EC1.14.15.4, EC1.14.15.5; UniProt P19099; also known as ALDOS, Aldosterone synthase, Aldosterone-synthesizing enzyme, CYPXIB2, Cytochrome P-450Aldo, Cytochrome P-450C18, Cytochrome P450 subfamily XIB polypeptide 2, Steroid 11-beta-monooxygenase, Steroid 11-beta/18-hydroxylase, Mitochondrial cytochrome P450 family 11 subfamily B polypeptide 2) is encoded by the CYP11B2 (Also known as CPN2, CYP11B, CYP11BL, P450C18) gene in human (Gene ID 1585). CYP11B2 protein is a member of the cytochrome P450 superfamily of monooxygenases that catalyze reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. CYP11B2 protein is localized to the mitochondrial inner membrane and exhibits steroid 18-hydroxylase activity and steroid 11 beta-hydroxylase activity. CYP11B2 gene mutations are known causes of corticosterone methyl oxidase 1 (CMO-1) deficiency, an autosomal recessive disorder of aldosterone biosynthesis.

Immunogen

Chicken serum albumin-conjugated linear peptide corresponding to human CYP11B2 near the N-terminus.
Epitope: Near N-terminus

Application

Anti-CYP11B2 Antibody, clone 41-17B is an antibody against CYP11B2 for use in Western Blotting, Immunohistochemistry, Immunofluorescence.
Immunohistochemistry Analysis: A representative lot detected CYP11B2 in normal adrenal glands (Gomez-Sanchez, C.E., et al. (2014). Mol. Cell Endocrinology. 383:111-117).
Immunofluorescence Analysis: A representative lot detected CYP11B2 in normal adrenal glands (Gomez-Sanchez, C.E., et al. (2014). Mol. Cell Endocrinology. 383:111-117).
Research Category
Signaling
Research Sub Category
Signaling Neuroscience

Quality

Evaluated by Western Blotting in hCYP11B2-GFP-expressing HEK293 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected CYP11B2 in 10 µg of hCYP11B2-GFP-expressing HEK293 cell lysate.

Target description

~57 kDa observed

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Kei Omata et al.
Hypertension (Dallas, Tex. : 1979), 72(4), 874-880 (2018-10-26)
Primary aldosteronism affects ≈5% to 10% of hypertensive patients and has unilateral and bilateral forms. Most unilateral primary aldosteronism is caused by computed tomography-detectable aldosterone-producing adenomas, which express CYP11B2 (aldosterone synthase) and frequently harbor somatic mutations in aldosterone-regulating genes. The
Chuanming Xu et al.
Acta physiologica (Oxford, England), 237(1), e13899-e13899 (2022-10-21)
The kaliuretic action of the renin-angiotensin-aldosterone system (RAAS) is well established as highlighted by hyperkalemia side effect of RAAS inhibitors but such action is usually ascribed to systemic RAAS. The present study addresses the involvement of intrarenal RAAS in K+
Jimmy J Mao et al.
Journal of the Endocrine Society, 5(8), bvab107-bvab107 (2021-07-15)
The detection and management of concomitant pheochromocytoma (PHEO) and primary aldosteronism (PA) is not well understood. To investigate varying presentations and outcomes of cases with coexisting PHEO and PA to provide an approach to its diagnosis and management. We conducted
Wenjuan Liu et al.
Acta pharmaceutica Sinica. B, 12(1), 135-148 (2022-02-08)
Hyperaldosteronism is a common disease that is closely related to endocrine hypertension and other cardiovascular diseases. Cytochrome P450 11B2 (CYP11B2), an important enzyme in aldosterone (ALD) synthesis, is a promising target for the treatment of hyperaldosteronism. However, selective inhibitors targeting
Kazutaka Nanba et al.
The Journal of clinical endocrinology and metabolism, 103(10), 3869-3876 (2018-08-08)
Somatic mutations have been identified in more than half of aldosterone-producing adenomas (APAs) through mutation hotspot sequencing. The underlying pathogenesis of inappropriate aldosterone synthesis in the remaining population is still unknown. To investigate the prevalence and spectrum of somatic mutations

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