MABS2028
Anti-Vitamin D receptor Antibody, clone 9A7
clone 9A7, from rat
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VDR, 1, 25-dihydroxyvitamin D3 receptor, Nuclear receptor subfamily 1 group I member 1
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biological source
rat
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
9A7, monoclonal
species reactivity
chicken, rat, porcine, human
packaging
antibody small pack of 25 μg
technique(s)
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
target post-translational modification
unmodified
Gene Information
chicken ... Vdr(395988)
human ... VDR(7421)
General description
Vitamin D3 receptor (UniProt: O42392; also known as VDR, 1,25-dihydroxyvitamin D3 receptor, Nuclear receptor subfamily 1 group I member 1) is encoded by the VDR (also known as NR1I1) gene (Gene ID: 395988) in chicken. VDR is a nuclear receptor for calcitriol, the active form of vitamin D3 that mediates the action of vitamin D on cells. VDR is expressed mainly in kidney and intestine and plays a central role in calcium homeostasis. In the absence of bound vitamin D3 it is present as a homodimer and it enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR. The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes. It is recruited to promoters via its interaction with BAZ1B/WSTF, which mediates the interaction with acetylated histones that is considered to be an essential step for VDR-promoter association. Plays a central role in calcium homeostasis. VDR is composed of three domains: a modulating N-terminal domain, a DNA-binding domain, and a C-terminal ligand-binding domain. Two isoforms of VDR have been described that are produced by alternative initiation.
Specificity
Clone 9A7 detects Vitamin D3 receptor in multiple avian and mammalian species.
Immunogen
Partially purified chicken intestinal vitamin D receptor.
Application
Anti-Vitamin D receptor, clone 9A7, Cat. No. MABS2028, is a rat monoclonal antibody that detects Vitamin D3 receptor and has been tested for use in , Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Immunoprecipitation Analysis: A representative lot detected Vitamin D receptor in Immunoprecipitation applications (Pike, J.W., et. al. (1982). Proc Natl Acad Sci USA. 79(24); 7719-23).
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Vitamin D receptor in human skin and human breast cancer tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Vitamin D receptor in human skin and human breast cancer tissue sections.
Research Category
Signaling
Signaling
Quality
Evaluated by Western Blotting in MCF-7 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Vitamin D receptor in MCF-7 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Vitamin D receptor in MCF-7 cell lysate.
Target description
~51 kDa observed; 51.3 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Linkage
Replaces: 04-1526
Physical form
Format: Purified
Protein G purified
Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
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Cell death & disease, 10(12), 936-936 (2019-12-11)
Chemoresistance is a major cause of cancer progression and the mortality of cancer patients. Developing a safe strategy for enhancing chemosensitivity is a challenge for biomedical science. Recent studies have suggested that vitamin D supplementation may decrease the risk of
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