MABS2297

Anti-TPO Antibody, clone 47

• Synonym(s): MoAb47, Thyroid peroxidase
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 47, monoclonal
• mol wt: calculated mol wt 102.96 kDa; observed mol wt ~120 kDa
• purified by: using protein G
• species reactivity: human
• packaging: antibody small pack of 100
• technique(s): ELISA: suitable; radioimmunoassay: suitable; western blot: suitable
• isotype: IgG1κ
• epitope sequence: Unknown
• Protein ID accession no.: NP_000538
• UniProt accession no.: P07202
• storage temp.: 2-8°C
• Gene Information: human ... TPO(7173)

Description

Specificity

Clone 47 is a mouse monoclonal antibody that specifically detects Thyroid peroxidase.

Immunogen

Human thyroid peroxidase (hTPO) purified from thyroid microsomes by immunoaffinity chromatography.

Application

Quality Control Testing

Evaluated by Western Blotting in human thyroid tissue extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Thyroid peroxidase (TPO) in human thyroid tissue extract.

Tested Applications

ELISA Analysis: A representative lot detected Thyroid peroxidase (TPO) in ELISA applications (Ruf, J., et al. (1989). Endocrinology. 125(3):1211-8).

Radioimmunoassay: A representative lot detected Thyroid peroxidase (TPO) in Radioimmunoassay applications (Ruf, J., et al. (1989). Endocrinology. 125(3):1211-8).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Thyroid peroxidase (Uniprot: P07202; also known as TPO) is encoded by gene TPO (Gene ID: 7173) in human. It is a single-pass type I membrane protein and has an Extracellular domain (19-846 aa), a Transmembrane domain ( 847-871 aa) and a Cytoplasmic domain (872-933 aa). It is primarily found in the thyroid gland and functions in the iodination of tyrosine residues in thyroglobulin and the formation of phenoxy-ester between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine (T3 and T4). TPO is an integral part of the complex process by which the thyroid gland regulates metabolism and other vital bodily functions. Defects in this gene can cause congenital hypothyroidism, congenital goitre and thyroid hormone organification defect IIA. Moreover, TPO is a common target of autoimmune attacks in various thyroid disorders, such as Hashimoto′s thyroiditis and Graves′ disease, which can lead to disruptions in hormone synthesis and subsequent health issues.
(Ref.: Ruf, J., et al. (1989). Endocrinology. 125(3):1211-8. (PMID: 2474433).

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable