MABT1514

Anti-Tenascin-R Antibody, clone 23-14

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 23-14, monoclonal
• mol wt: calculated mol wt 148.28 kDa; observed mol wt ~170 kDa
• purified by: using protein G
• species reactivity: human, mouse, chicken, rat
• packaging: antibody small pack of 100 μg
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; inhibition assay: suitable; western blot: suitable
• isotype: IgG1κ
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_990607
• UniProt accession no.: Q00546
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: chicken ... TNR(396213)

Description

General description

Tenascin-R (UniProt: Q00546; also known as TN-R, Restrictin) is encoded by the TNR gene (Gene ID: 396213) in chicken. Tenascins are a family of four large glycoproteins (Tenascin-C, R, X, and W) that share a cysteine-rich N-terminus, a sequence of fibronectin type III domains, and homologies to fibronectin-b at the C-terminus. Tenascin-R (TN-R), the smallest member of the tenascin family that can exist as homodimer or homotrimer. It interacts with different cells and matrix components and is involved in neural cell adhesion and neurite formation. Its interaction with contactin-1 enhances neurite outgrowth. It is expressed weakly in E6 embryo and reaches a maximum at E16 and declines in the adult. Its expression is observed in oligodendrocytes during myelination, and it accumulates at Ranvier nodes and by a subpopulation of neurons, including cerebellar stellate/basket cells, motoneurons in the spinal cord, and hippocampal interneurons. TN-R is synthesized with a signal peptide (aa 1-33), which is subsequently cleaved off to generate the mature form. Its N-terminal region contains a cysteine-rich segment that it shares with other members of tenascin family. Within this segment there is a stretch of 20 amino acids that form three heptad repeats of hydrophobic amino acids in an a-helix that might generate a triple-stranded coiled coil to link three or two TN-R polypeptides to trimers or dimers. It also contains four EGF-like domains and nine fibronectin type-III (Fn-III) domains. Fn-III 9 mediates cell attachment and Fn-III 2-5 are reported to mediate neurofascin binding. It also contains a fibrinogen C-terminal domain (aa 1124-1339) that mediates its interaction with chondroitin Sulfate Proteoglycan 5. (Ref.: Nörenberg, U., et al. (1995). J. Cell Biol. 130(2); 473-484; Rathjen, FG., et al. (1991). Development.113(1); 151-164).

Specificity

Clone 23-14 is a mouse monoclonal antibody that detects Tenascin-R. It targets an epitope within the Fibronectin type-III 8 domain from the C-terminal half.

Immunogen

Immunoaffinity isolate of the monoclonal antibody F11 from detergent extracts of adult chicken brain plasma membrane preparations.

Application

Quality Control Testing

Evaluated by Western Blotting in Rat brain tissue lysates.

Western Blotting Analysis: A 1:125 dilution of this antibody detected Tenascin-R in Rat brain tissue lysates.

Tested Applications

Inhibition Assay: A representative lot of this antibody inhibited neural cell and retinal cell attachment to Tenascin-R. (Rathjen, F.G., et al. (1991). Development. 113(1):151-64; Norenberg, U., et al. (1995). J Cell Biol. 130(2):473-84; Norenberg, U., et al. (1996). Int J Dev Neurosci. 14(3):217-31).

Immunohistochemistry (Paraffin) Analysis: A 1:50-250 dilution from a representative lot detected Tenascin-R in mouse brain and rat brain tissue sections.

Western Blotting Analysis: A representative lot detected Tenascin-R in Western Blotting applications (Wintergerst, E.S., et al. (2001). J Neurocytol. 30(4):293-301).

Immunohistochemistry Analysis: A representative lot detected Tenascin-R in Immunohistochemistry applications (Haunso, A., et al. (2000). Brain Res.;864(1):142-5; Wintergerst, E.S., et al. (2001). J Neurocytol.;30(4):293-301; Geissler, M., et al. (2013). J Neurosci.;33(18):7742-55).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Tenascin-R, clone 23-14, Cat. No. MABT1514, is a mouse monoclonal antibody that detects Tenascin-R and is tested for use in Immunohistochemistry (Paraffin), Inhibition Assay, and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in PBS without azide.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable