Skip to Content
MilliporeSigma
All Photos(2)

Documents

MABT328

Sigma-Aldrich

Anti-Siglec-1 (CD169) Antibody, clone 5F1.1

clone 5F1.1, from mouse

Synonym(s):

Sialoadhesin, Sialic acid-binding Ig-like lectin 1, Siglec-1, CD169, Siglec-1 (CD169)

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5F1.1, monoclonal

species reactivity

human, mouse

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SIGLEC1(6614)

Related Categories

General description

Sialoadhesin (UniProt Q9BZZ2; also known as CD169, Sialic acid-binding Ig-like lectin 1, Sialic acid-binding immunoglobulin-like lectin 1, Siglec-1) is encoded by the SIGLEC1 (also known as SN) gene (Gene ID 6614) in human. Sialoadhesin/CD169/Siglec-1 is the founding member of the sialic acid-binding immunoglobulin lectins (Siglec) expressed on a variety of leukocytes and stromal cells. Sialoadhesin is initially produced with a signal sequence (a.a. 1-19), the removal of which yields the mature protein with a large extracellular portion (a.a. 20-1641), followed by a transmembrane segment (a.a. 1642-1662), and a short cytoplasmic tail (a.a. 1663-1709) with no known signal transduction or endocytosis motifs. The extracellular portion contains an N-terminal Ig-like V-type domain (a.a. 20-136), followed by sixteen Ig-like C2-type domains. Sialoadhesin is highly expressed on the subcapsular sinus (SCS) and medullary macrophages in lymph node (LN) and on marginal metallophilic macrophages in the marginal zone (MZ) of spleen. Although sialic acids are found on the surface of all cells and most secreted proteins, Only heavily sialylated, multimeric structures are captured by Sialoadhesin on macrophages via its N-terminal Ig-like V-type domain due to a low (millimolar) affinity toward sialylic acid. Sialoadhesin-mediated sialic-acid recognition allows macrophages to engage sialylated pathogens and stimulate inflammatory responses. Sialoadhesin is also reported to capture of B cell-derived exosomes via their surface-expressed α2, 3-linked sialic acids. In the lymph node, exosomes are not retained in the subcapsular sinus of Siglec1-knockout mice and penetrate deeper into the paracortex, causing an enhanced response to antigen-pulsed exosomes among Siglec1-/- mice.

Specificity

Expected to react with all three spliced isoforms of human Siglec-1 (CD169)/Sialoadhesin reported by UniProt (Q9BZZ2).

Immunogen

Epitope: Ig-like V-type domain
GST-tagged recombinant human Siglec-1 (CD169) N-terminal fragment containing the Ig-like V-type domain.

Application

Anti-Siglec-1 (CD169) Antibody, clone 5F1.1 is an antibody against Siglec-1 for use in Western Blotting, Immunohistochemistry (Paraffin).
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Siglec-1 (CD169) in human spleen, human tonsil, and mouse spleen tissue.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Quality

Evaluated by Western Blotting in human lymph node tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Siglec-1 (CD169) in 10 µg of human lymph node tissue lysate.

Target description

~200 kDa observed. Target band size appears larger than the calculated molecular weights of 182.6 kDa (isoform 1), 175.7 kDa (isoform 2), and 181.7 kDa (isoform 3) due to glycosylation.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Still not finding the right product?  

Give our Product Selector Tool a try.

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Elise Siegert et al.
Acta neuropathologica, 141(6), 917-927 (2021-04-18)
Systemic sclerosis represents a chronic connective tissue disease featuring fibrosis, vasculopathy and autoimmunity, affecting skin, multiple internal organs, and skeletal muscles. The vasculopathy is considered obliterative, but its pathogenesis is still poorly understood. This may partially be due to limitations
Luca Cassetta et al.
Cancer cell, 35(4), 588-602 (2019-04-02)
The roles of tumor-associated macrophages (TAMs) and circulating monocytes in human cancer are poorly understood. Here, we show that monocyte subpopulation distribution and transcriptomes are significantly altered by the presence of endometrial and breast cancer. Furthermore, TAMs from endometrial and
Kiera L Clayton et al.
Cell host & microbe, 29(3), 435-447 (2021-02-12)
Natural killer (NK) cells are innate cytolytic effectors that target HIV-infected CD4+ T cells. In conjunction with antibodies recognizing the HIV envelope, NK cells also eliminate HIV-infected targets through antibody-dependent cellular cytotoxicity (ADCC). However, how these NK cell functions impact infected

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service