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MABT902

Sigma-Aldrich

Anti-Connexin 43 Antibody, clone P2C4

clone P2C4, from mouse

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Synonym(s):
Gap junction alpha-1 protein, CX43, Gap junction 43 kDa heart protein
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

P2C4, monoclonal

species reactivity

human, rat, mouse

species reactivity (predicted by homology)

canine (based on 100% sequence homology), hamster (based on 100% sequence homology), bovine (based on 100% sequence homology), porcine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), rabbit (based on 100% sequence homology), guinea pig (based on 100% sequence homology)

technique(s)

electron microscopy: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

bovine ... Gja1(281193)
dog ... Gja1(403418)
guinea pig ... Gja1(100379273)
human ... GJA1(2697)
mouse ... Gja1(14609)
rabbit ... Gja1(100008935)
rat ... Gja1(24392)
rhesus monkey ... Gja1(714344)

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vibrant-m

AB1727

Anti-Connexin 43 Antibody

clone

P2C4, monoclonal

clone

P4G9, monoclonal

clone

P1E11, monoclonal

clone

polyclonal

species reactivity

human, rat, mouse

species reactivity

mouse, human, rat

species reactivity

mouse, rat, human

species reactivity

mouse, bovine, rat

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

isotype

IgG2aκ

isotype

IgG2aκ

isotype

IgG1κ

isotype

-

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General description

Gap junction alpha-1 protein (UniProt: P08050; also known as Connexin-43, CX43, Gap junction 43 kDa heart protein) is encoded by the Gja1 (also known as Cxn-43) gene (Gene ID: 24392) in rat. The gap junction channels are dodecamers of transmembrane proteins of the connexin family. Connexins usually have a short half-life ( 1.5 5 h) and this short half-life is a key element of coupling regulation as it allows very dynamic and acute changes in gap junction regulation and gap junctional communication. In cardiomyocytes three types of connexins have been identified: Connexin 40, 43, and 45. Each of these connexins form channels with unique and specific electrophysiological properties. Connexin-43 is a multi-pass membrane protein and is the most ubiquitous connexin with widespread tissue expression pattern. Connexin-43 can be phosphorylated at multiple sites and phosphorylation is shown to regulate the kinetics of its trafficking, assembly, gating and turnover in a cell-cycle-stage specific manner. Phosphorylation at Ser325, Ser328 and Ser330 by casein kinase I is reported to modulate gap junction assembly while phosphorylation of Ser368 by protein kinase C gamma isoform leads to disassembly of gap junction plaques and inhibition of gap junction activity. During myocardial ischemia connexin-43 is shown to become dephosphorylated that allows gap junctions to be opened and convey the propagation of ischemia injury. In bladder smooth muscle cells, connexin-43 displays a circadian cycle with low levels during the sleep phase.

Specificity

Clone P2C4 detects Connexin 43 in human heart. It targets an epitope within 23 amino acids from the C-terminal region. It primarily binds to the non-phosphorylated isoform around Ser364/365.

Immunogen

KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal region of rat connexin 43.

Application

Anti-Connexin 43 Antibody, clone P2C4, Cat. No. MABT902, is a mouse monoclonal antibody that targets Connexin-43 and is tested for use in Electron Microscopy, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Connexin 43 in rat heart tissue.

Immunoprecipitation Analysis: A representative lot detected Connexin 43 in Immunoprecipitation applications (Solan, J.L., et. al. (2003). J Cell Sci. 116(Pt 11):2203-11).

Electron Microscopy Analysis: A representative lot detected Connexin 43 in Electron Microscopy applications (Sosinsky, G.E., et. al. (2007). Biochem J. 408(3):375-85).

Immunocytochemistry Analysis: A representative lot detected Connexin 43 in Immunocytochemistry applications (Sosinsky, G.E., et. al. (2007). Biochem J. 408(3):375-85).

Western Blotting Analysis: A representative lot detected Connexin 43 in Western Blotting applications (Sosinsky, G.E., et. al. (2007). Biochem J. 408(3):375-85).

Quality

Evaluated by Immunohistochemistry in human heart tissue.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Connexin 43 in human heart tissue.

Target description

43.03 kDa calculated.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Shaohua Xiao et al.
The Journal of clinical investigation, 130(9), 4858-4870 (2020-06-12)
Connexin-43 (Cx43) gap junctions provide intercellular coupling, which ensures rapid action potential propagation and synchronized heart contraction. Alterations in Cx43 localization and reductions in gap junction coupling occur in failing hearts, contributing to ventricular arrhythmias and sudden cardiac death. Recent

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