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MAC141

Sigma-Aldrich

Anti-Cas9 Antibody, C-terminal Antibody, clone 5F1 Antibody, S. aureus

clone 5F1, from mouse

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Synonym(s):
SaCas9, CRISPR-associated endonuclease Cas-9
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

5F1, monoclonal

species reactivity

Staphylococcus aureus, bacteria

species reactivity (predicted by homology)

all

packaging

antibody small pack of 25 μg

technique(s)

western blot: suitable

isotype

IgG1κ

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

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MAC142MAB131872ABS2202
Anti-Cas9 Antibody, clone 6F7 clone 6F7, from mouse

MAB131872

Anti-Cas9 Antibody, clone 6F7

Anti-Cas9 from rabbit, purified by affinity chromatography

ABS2202

Anti-Cas9

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

species reactivity

Staphylococcus aureus, bacteria

species reactivity

Staphylococcus aureus, bacteria

species reactivity

Staphylococcus aureus, mouse

species reactivity

Streptococcus pyogenes, bacteria

clone

5F1, monoclonal

clone

6H4, monoclonal

clone

6F7, monoclonal

clone

polyclonal

isotype

IgG1κ

isotype

IgG2bκ

isotype

IgG1κ

isotype

-

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

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General description

CRISPR-associated endonuclease Cas9 (UniProt: J7RUA5; also known as SaCas9) is encoded by the Cas9 gene (Gene ID: in Staphylococcus aureus. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self-versus non-self, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity. Cas9 endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, and then trimmed 3′-5′ exonucleolytically.

Specificity

Clone 5F1 detects Cas-9 in Staphylococcus aureus.

Immunogen

Epitope: C-terminus
His-tagged recombinant Cas-9 from Staphylococcus aureus.

Application

Anti-Cas-9, C-terminal, clone 5F1, Cat. No. MAC141, is a mouse monoclonal antibody that detects Cas-9 in Staphylococcus aureus and its identity has been confimed by Isotyping Test and Western Blotting.
Research Category
Secondary & Control Antibodies
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Cas-9 in HEK293/Cas9 from S. aureus or HEK293 (WT) cell lysates. (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).

Quality

Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .

Target description

~124 kDa observed; 123.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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