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MGAMMAG-300E

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MILLIPLEX® Mouse IgE Single Plex Magnetic Bead Kit - Isotyping Multiplex Assay

Isotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulin E (IgE) in mouse serum, plasma and cell culture samples.

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47

Quality Level

200

species reactivity

mouse

manufacturer/tradename

Milliplex®

assay range

accuracy: 82%
sensitivity: 0.16 ng/mL
standard curve range: 0.8-3500 ng/mL
inter-assay cv: <10%
intra-assay cv: <5%

technique(s)

multiplexing: suitable

compatibility

configured for Single Plex

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

General description

Found only in mammals, Immunoglobulin E (IgE) plays an essential role in allergic reactions and is predominantly associated with type 1 hypersensitivity. IgE is the least abundant immunoglobulin isotype in normal or topic mammals, present at only 0.05% of IgG serum concentrations. It can trigger robust immune reactions, with up to 10 times the typical level detected in the serum of atopic mammals, although increased levels occur only in approximately 50% of mammals with allergic disorders. IgE binds with high affinity to FcRI, found primarily on mast cells and basophils, priming the cells to recognize specific antigens. Once primed and with repeated exposure to the same antigen, these cells release molecules such as histamines, prostaglandins, and leukotrienes, resulting in an “allergic reaction”.

The MILLIPLEX® Mouse IgE Single Plex Magnetic Bead Kit is a single plex kit to be used to measure levels of IgE in only 2 μL of mouse urine, serum, plasma or tissue/cell lysate and culture supernatant samples.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Immune Response

Specificity

Cross-reactivity between these antibodies and those against other immunoglobulins is non-detectable or negligible.

Application

  • Analyte: IgE
  • Recommended Sample type: Serum, plasma, tissue/cell lysates and tissue culture supernatant
  • Recommended Sample dilution: 1:200 dilution for plasma or serum. Tissue/ cell culture supernatants are diluted to approximately 1μg/mL
  • Assay Run Time: One day
  • Research Category: Immunology and Inflammation, Isotyping

Packaging

96 Well Plate

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Syed H Haider et al.
American journal of respiratory cell and molecular biology, 63(2), 219-233 (2020-04-22)
Pulmonary disease after World Trade Center particulate matter (WTC-PM) exposure is associated with dyslipidemia and the receptor for advanced glycation end products (RAGE); however, the mechanisms are not well understood. We used a murine model and a multiomics assessment to
Tsvetana Petrova et al.
PloS one, 17(2), e0263151-e0263151 (2022-02-15)
The expression of TNF-Receptor Associated Factor 6 (TRAF6) is essential for many physiological processes. Here we studied the phenotype of TRAF6[L74H] knock-in mice which are devoid of TRAF6 E3 ligase activity in every cell of the body, but express normal
Kea Martin et al.
Journal of immunology (Baltimore, Md. : 1950), 203(11), 2791-2806 (2019-10-30)
The paracaspase Malt1 is a key regulator of canonical NF-κB activation downstream of multiple receptors in both immune and nonimmune cells. Genetic disruption of Malt1 protease function in mice and MALT1 mutations in humans results in reduced regulatory T cells

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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