PBGC06210
PES Membrane Filter, 10 kDa NMWCO
Biomax®, filter diam. 63.5 mm
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Biomax PES ultrafiltration membrane discs, Ultrafiltration Discs, 10 kDa NMW
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material
polyethersulfone membrane
Quality Level
manufacturer/tradename
Biomax®
technique(s)
protein clean-up: suitable (desalting)
protein purification: suitable (concentration)
filter diam.
63.5 mm
filtration area
28.7 cm2
pore size
10 kDa NMWCO
shipped in
ambient
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General description
Ultrafiltration membranes are typically made from regenerated cellulose or polyethersulfone (PES). The material of choice will greatly depend on sample compatibility. The choice of membrane nominal molecular weight cut-off (NMWCO) will depend on the molecular weight (MW) of the macrosolute that is to be retained. Membrane choice will have a significant impact on performance. As a rule, the NMWCO should be 2-3 times smaller than the molecular weight of the solute to be retained when using regenerated cellulose and 4-5 times smaller for Biomax® PES membranes.,Diafiltration uses ultrafiltration membranes, either in centrifugal or pressure-driven devices such as the Amicon® Stirred Cell for efficient buffer exchange. Unlike dialysis tubing, ultrafiltration membranes can be used for both sample concentration and buffer exchange, thus minimizing sample transfers and reducing sample loss.
Application
Ultrafiltration Discs, 10 kDa NMW has been used in the ultrafiltration unit for Schizosaccharomyces japonicus polysaccharides recovery and purification following alcoholic fermentation.
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
BIOMAX is a registered trademark of Merck KGaA, Darmstadt, Germany
wgk_germany
WGK 1
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Foods (Basel, Switzerland), 9(10) (2020-10-08)
Nowadays commercial preparations of yeast polysaccharides (PSs), in particular mannoproteins, are widely used for wine colloidal and tartrate salt stabilization. In this context, the industry has developed different processes for the isolation and purification of PSs from the cell wall
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