MilliporeSigma
All Photos(6)

Documents

PTHT12H48

Millipore

Millicell® 12 well hanging cell culture inserts

pore size 0.4 μm, translucent PET membrane, diam. 12 mm, Tissue Culture (TC)-treated surface, sterile

Sign Into View Organizational & Contract Pricing

Synonym(s):
cell culture inserts, permeable culture insert, plate inserts, tissue culture insert, tissue culture plate
eCl@ss:
32011202

material

polystyrene housing
translucent PET membrane

Quality Level

sterility

γ-irradiated
sterile

feature

100x106 pores/cm2

packaging

pack of 48 ea (individually blister packaged)

manufacturer/tradename

Millicell®

parameter

37 °C max. temp.

H

15.5 mm

diam.

12 mm

insert maximum volume

0.8 mL

insert working volume

0.4 mL

size

12 wells

surface area

1.1 cm2

color

translucent membrane

pore size

0.4 μm

binding type

Tissue Culture (TC)-treated surface

General description

Millicell® Hanging Cell Culture inserts with Polyethylene Terephthalate (PET) membrane are ideal for the growth and differentiation of various cell types. This individually sterile-packaged insert has a 0.4 μm membrane pore size and fits in most 12-well plastic tissue culture plates.

In plastic tissue culture plates, cells can access media only from their apical sides, but in Millicell® tissue culture inserts, cells can access media from both their apical and basolateral sides. As a result, cell growth, structure, and function more closely mimic what occurs in vivo.

The insert’s flanged design suspends it within the culture plate well and makes it possible to access both sides of the cell monolayer. Spaces between three protruding arms facilitate pipetting between the outside of the insert and the culture plate well.

Application

  • Cell Proliferation
  • Cell Surface Receptors
  • Co-Culture
  • Epithelial Cell Growth
  • Feeder Layers
  • Fluorescent Detection / Immunohistochemistry
  • In Vitro Toxicology
  • Microbial Attachment
  • Polarized Protein Secretions
  • Polarized Uptake
  • Transport / Permeability

Legal Information

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Articles

How to set up barrier formation and permeability assays using cell culture inserts and TEER measurements.

16HBE14o- human bronchial epithelial cells used to model respiratory epithelium for the research of cystic fibrosis, viral pulmonary pathology (SARS-CoV), asthma, COPD, effects of smoking and air pollution. See over 5k publications.

Protocols

3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service