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SCC043

Sigma-Aldrich

C28/I2 Human Chondrocyte Cell Line

C28/I2 Human Chondrocyte Cell Line is widely used as a model cell line for studying normal and pathological cartilage repair mechanisms related to chondrocyte biology and physiology.

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Synonym(s):
C-28/I2, C28/I2, Human chondrocyte cell line
eCl@ss:
32011203

biological source

human

Quality Level

technique(s)

cell culture | mammalian: suitable

shipped in

ambient

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SCC041SCC064SCC109
technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | mammalian: suitable

shipped in

ambient

shipped in

ambient

shipped in

liquid nitrogen

shipped in

dry ice

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

The chondrocyte is the only specialized cell type present in articular cartilage. They produce secretions to support and repair the cartilage matrix. Human chondrocytes in mature articular cartilage are post-mitotic and terminally differentiated cells. Studies on human chondrocytes have been hampered by the difficulty in obtaining sufficient numbers of primary human chondrocytes from a single joint and by variabilities among donors due to factors such as age and medical conditions.

C-28/I2 is a clonal line derived from the nonclonal T/C-28a4 cells which were immortalized via retroviral vector-mediated simian virus SV40 Large T antigen (Tag) expression.

Cell Line Description

Chondrocyte Cells

Application

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Quality

• Each vial contains ≥ 1X106 viable cells.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.

Storage and Stability

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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25G
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Sex hormones and insulin have been implicated in articular cartilage metabolism. To supplement previous findings on the regulation of matrix synthesis with 17β-estradiol and insulin and to find a possible model to study cartilage metabolism in vitro, we evaluated the

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