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3dGRO® Human CRC Organoids (ISO34)

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Synonym(s):
organoid, PDO, cancer organoid, tumor organoid, 3d cell culture, colorectal cancer

biological source

human

Quality Level

packaging

vial of ≥100,000 organoids/vial vial

manufacturer/tradename

Millipore

growth mode

N/A

technique(s)

cell culture | stem cell: suitable

shipped in

dry ice

storage temp.

-65 to -96°C

General description

Colorectal cancer accounts for roughly 10% of all cancer cases worldwide with more than half of all patients with CRC developing metastatic disease leading to death. Recently, tissue derived organoids have emerged as a more predictive 3 dimensional cell culture model of disease. 3D organoid cultures conserve the original genetic and phenotypic characteristics of the primary tissue allowing for their application in many research fields included drug development, personalized medicine and potential therapeutics. In vitro cultured tumor organoids have also been shown to predict patient response to chemotherapeutics. PDOs derived from colorectal cancer (CRC organoids) have been used for cell modeling and to investigate the function of cancer related driver gene mutations including APC, TP53, KRAS, BRAF, PIK3CA etc.

3dGRO® Human CRC Organoids are offered in a ready-to-assay format intended to simplify the organoid cell culture workflow. A single vial of organoids is enough to directly thaw and seed into a 96-well plate without cell expansion for drug screening applications. The organoids are manufactured using Cellesce′s patented technology which enables the robust growth and expansion of patient-derived organoids (PDOs). Cellesce′s technology minimizes manual handling time to maximize reproducibility in order to position organoid cell models as a cost effective and accurate tool in early-stage drug discovery. The organoid biobank includes tumor derived colorectal cancer PDOs from a range of genetic backgrounds, driver gene mutations, tumor sites and cancer stages. These organoid cell lines have been well characterized and are all validated for response against a number of known CRC-targeting agents.

Application

Cell Growth: Organoid expansion after 7 days
Mycoplasma: Negative
Viral Testing: Negative (HIV-1, HIV-2, HBV, CMV, EBV, HPV)
Sterility (Bacteria, Yeast, Fungi): Negative
Mutational Profile: APC ͰE1451*, BRAF ͰK601E

Features and Benefits

Ready-to-Assay human colorectal cancer organoids for 3D cell culture applications.

Storage and Stability

Store 3dGRO® Human CRC Organoids at -80°C. For long term storage the cells should be stored in liquid nitrogen.

Legal Information

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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Luned M Badder et al.
PloS one, 15(8), e0235319-e0235319 (2020-08-19)
Aberrant activation of the Wnt signalling pathway is required for tumour initiation and survival in the majority of colorectal cancers. The development of inhibitors of Wnt signalling has been the focus of multiple drug discovery programs targeting colorectal cancer and

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