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SCT021

Sigma-Aldrich

BioTracker 609 Red Ca2+ AM Dye

Live cell imaging calcium (Ca2+) indicator for neuroscience and cancer applications.

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Synonym(s):
Live cell calcium detection probe, Live cell imaging probe, Red fluorescent calcium indicator
eCl@ss:
32161000

technique(s)

cell based assay: suitable

detection method

fluorometric

shipped in

ambient

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SCT021

BioTracker 609 Red Ca2+ AM Dye

vibrant-m

SCT119

BioTracker 650 Red Nuclear Dye

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BioTracker 640 Red C1 Synaptic Dye

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

detection method

fluorometric

detection method

fluorometric

detection method

fluorometric

detection method

fluorometric

General description

Calcium (Ca2+) play a vital role in the physiology and biochemistry of the cell. Calcium ions play an important role in signal transduction pathways, neurotransmitter release from neurons, contraction of all muscle cell types, and in fertilization. Extracellular calcium is also important for maintaining the potential difference across excitable cell membranes, as well as proper bone formation.

The BioTracker 609 Red Ca2+ AM Dye is a live cell red fluorescent calcium indicator. Fluorescence intensity of this fluorescent probe reversibly changes depending on Ca2+ concentration. It has red fluorescence with maximum emission at 609 nm (Excitation 590nm) and is suitable for detection of micromolar- to submicromolar-range calcium ions. The dye is hydrolyzed by intracellular esterases to become soluble which equally distributes in the cytoplasm. This feature enables detection of intracellular calcium concentration changes with superior accuracy compared to other calcium indicators.

Spectral Properties
Absorbance: 595nm
Emission: 609nm

Storage and Stability

Store BioTracker 609 Red Ca2+ AM Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

1202.4 °F

flash_point_c

650.2 °C


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