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SCT129

Sigma-Aldrich

BioTracker 640 Red C2M(FM5-95) Synaptic Dye

Live cell imaging synaptic dye that detects neural synaptic activitiy at neuromuscular junctions and synapses.

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Synonym(s):
Live cell imaging probe

technique(s)

cell based assay: suitable

detection method

fluorometric

shipped in

ambient

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SCT127SCT130SCT126
shipped in

ambient

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wet ice

shipped in

ambient

shipped in

ambient

detection method

fluorometric

detection method

fluorometric

detection method

fluorometric

detection method

fluorometric

General description

BioTracker synaptic dyes are a series of fluorescent cationic styryl dyes developed to follow synaptic activities at neuromuscular junctions or synapses. These dyes typically have a lipophilic tail at one end and a highly hydrophilic at the other end. In the presence of cells or tissue preparations, the dyes partition between the aqueous phase, where the dyes are virtually non-fluorescent, and the outer leaflet of the cell surface membranes, where the dyes insert the lipophilic end into the membranes and become intensely fluorescent. During endocytosis following nerve stimulation, the dyes become trapped inside the vesicles. Thus, after washing off the dyes on the cell surface, the fluorescent signal is proportional to the number of newly formed vesicles. On the other hand, during exocytosis, the dyes are released from the vesicles along with neurotransmitters, causing a decrease in fluorescent signal. As a result, the change in fluorescent intensity reflects the amount of endocytosis/exocytosis or synaptic activity.

BioTracker 640 Red C2M(FM5-95) Synaptic Dye is a red fluorescent probe used to follow synaptic vesicles. The dye is slightly more water soluble than BioTracker 640 Red C2(FM4-64) Synaptic Dye.

Spectral Properties
Absorbance (Membranes): 510 nm
Emission (Membranes): 750 nm

Application

Live cell fluorescent imaging
Live cell imaging synaptic dye that detects neural synaptic activitiy at neuromuscular junctions and synapses.
Research Category
Cell Imaging
Research Sub Category
Live Cell Dye

Quality


Spectral Properties
Absorbance (Membranes): 510 nm
Emission (Membranes): 750 nm

Physical form

Lyophilized

Storage and Stability

Store BioTracker 640 Red C2M(FM5-95) Synaptic Dye at 2-8°C, protected from light. Stock solutions can be prepared at 10 mM and stored at 4°C or -20°C for six months or longer.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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