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SF001

Sigma-Aldrich

ESGRO Complete PLUS Clonal Grade Medium, 100ml

The ESGRO Complete PLUS Clonal Grade Medium is a defined serum-free medium provided with a selective GSK3β inhibitor to enhance viability of mouse embryonic stem cells & increased maintenance of pluripotency in the absence of serum & feeder cells.

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Synonym(s):
Mouse ES Cell Media
eCl@ss:
32160801

Quality Level

100
300

form

liquid

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

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technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

Quality Level

100, 300

Quality Level

300

Quality Level

300

Quality Level

300

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

Chemicon®

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

input

sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type neural stem cell(s)

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

General description

ESGRO Complete PLUS Clonal Grade Medium is a defined serum-free medium provided with a selective GSK3β inhibitor to enhance viability of mouse embryonic stem (ES) cells and increased maintenance of pluripotency in the absence of serum and feeder cells. This medium is intended for mouse ES cell expansion and growth at clonal density in serum-free conditions. The mouse ES cells should maintain germ line competency.

Application

Suitable for ES cell derivation, transfection and selection, in addition to general cell passaging and growth.
Media can be used on feeder dependent/independent ES cell lines.

Important passaging information
Use of one of the below listed cell dissociation solutions is imperative while passaging ES cells using ESGRO Complete products. Standard trypsin protocols used to passage ES cells grown in serum containing media typically result in over trypsinization causing the cells to lift off the plates and form abnormal ball-like structures.
Recommended products include:
ESGRO Complete Accutase (Cat # SF006)
ESGRO Complete Trypsin Solution (proprietary) (Cat # SF007)
ESGRO Complete Enzyme-Free Dissociation Solution (proprietary) (Cat # SF009)

Components

1. ESGRO Complete Clonal Grade Medium: (Catalogue No. SF001-C) One (1) 100 mL bottle containing original clonal grade medium. Complete formulation containing self-renewal factors, no need to add LIF. pH 7.1-7.3

2. GSK3β Inhibitor Supplement: (Catalogue No. SF012-050) One (1) 50 µL vial containing selective GSK3β inhibitor.

Quality

QC PROTOCOL:

Low density plating assay:
1. Plate feeder independent early passage ES cells onto gelatin coated 10 cm plastic tissue culture dish at 1000 cells/10 cm dish in 10-20 mL of Clonal Grade Medium.
2. Change medium every 2-4 days.
3. Monitor for clonal growth with alkaline phosphatase stain.

Colony formation should occur over 4-6 days. The majority of colonies should be alkaline phosphatase positive and show no signs of differentiation.

Linkage

Replaces: SF001-100

Storage and Stability

• Upon receipt, store clonal grade medium and supplement in the dark at -20°C. Refer to lot expiration date on labels.
• Thawed clonal grade medium should be stored in the dark at 4°C for one month. Do not refreeze.
• Supplement may be thawed at room temperature, aliquoted and stored in the dark at 4°C for two months.
• After supplement is added to clonal grade medium, store in the dark at 4°C for up to
two weeks. Do not refreeze.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
ESGRO is a registered trademark of Merck KGaA, Darmstadt, Germany
cOmplete is a trademark of Roche

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Health hazard

signalword

Danger

Hazard Classifications

Lact. - Repr. 1A

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 2


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Dynamic transcriptional events in embryonic stem cells mediated by the super elongation complex (SEC).
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A critical appraisal of factors affecting the accuracy of results obtained when using flow cytometry in stem cell investigations: where do you put your gates?
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A potential use of embryonic stem cell medium for the in vitro culture of preimplantation embryos.
Gelber K. et al.
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Optimized mouse ES cell culture system by suspension growth in a fully defined medium.
Andang M. et al.
Nature Protocols null

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