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10988537001

Roche

7-Deaza-2′-deoxy-guanosine-5′-triphosphate

95% (7-Deaza-dGTP, HPLC), ≤4% (7-Deaza-dGDP, HPLC), solution, 10 mM

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Synonym(s):
7-Deaza-2′-deoxyguanosine 5′-triphosphate lithium salt, −N7-dGTP, 7-Deaza-dGTP
Empirical Formula (Hill Notation):
C11H17N4O13P3
CAS Number:
Molecular Weight:
506.19
MDL number:
PubChem Substance ID:

Quality Level

form

solution

composition

7-Deaza-dGDP, ≤4% HPLC
7-Deaza-dGTP, 95% HPLC

packaging

pkg of 200 μL (10 mM, 2 μmol)

manufacturer/tradename

Roche

concentration

10 mM

storage temp.

−20°C

SMILES string

NC1=NC(=O)c2ccn(C3CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O3)c2N1

InChI

1S/C11H17N4O13P3/c12-11-13-9-5(10(17)14-11)1-2-15(9)8-3-6(16)7(26-8)4-25-30(21,22)28-31(23,24)27-29(18,19)20/h1-2,6-8,16H,3-4H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,12,13,14,17)

InChI key

DLLXAZJTLIUPAI-UHFFFAOYSA-N

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1 of 4

This Item
B6396D0184D8783
manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

form

solution

form

powder

form

liquid

form

liquid

packaging

pkg of 200 μL (10 mM, 2 μmol)

packaging

-

packaging

-

packaging

-

concentration

10 mM

concentration

-

concentration

100 mM in H2O

concentration

10 mM in H2O

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

7-deaza-2′-deoxy-guanosine-5′-triphosphate is a nucleotide analog that is a candidate substrate for processive telomerase. The use of this analog improves PCR (polymerase chain reaction) yields. This compound also makes sequencing of GC rich sequences easier, especially if template amount is low and/or DNA quality is not good.
The product contains 7-Deaza-2′-deoxy-guanosine-5′-triphosphate as a 10 mM solution of the lithium salt.

Application

7-Deaza-dGTP is a substrate for most DNA polymerases, including Taq DNA polymerase.
7-Deaza-dGTP is used in the dideoxy-chain termination sequencing methods, in place of dGTP to overcome compression problems in gel electrophoresis when sequencing GC-rich stretches of DNA.
Partial substitution of 7-Deaza-dGTP for dGTP in PCRs can improve the product yield when the template is GC-rich (i.e., has extensive secondary structures).

Preparation Note

Working solution: Dilute 7-deaza-dGTP into a solution containing 50 mM Tris, pH 7.0, and use at exactly the same concentration as you would use dGTP.

Analysis Note

Absorption: 25 A260 units of product correspond to 1 mg.
Absorbance determination: A250/A260, A280/A260, A290/A260

Other Notes

When PCR products contain 7-Deaza dGTP, they do not stain well in ethidium bromide, presumably because stacking of adjacent bases in the products is diminished.
PCR amplification efficiency is generally not affected by 7-Deaza-dGTP.
For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

No data available

flash_point_c

No data available


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