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11426338910

Roche

Anti-Fluorescein-AP, Fab fragments

from sheep

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Synonym(s):
antibody

biological source

sheep

Quality Level

conjugate

alkaline phosphatase conjugate

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

form

solution

packaging

pkg of 150 U (200 μl)

manufacturer/tradename

Roche

isotype

IgG

storage temp.

2-8°C

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This Item
114263469101120774191011093274910
conjugate

alkaline phosphatase conjugate

conjugate

peroxidase conjugate

conjugate

fluorescein conjugate

conjugate

alkaline phosphatase conjugate

antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

solution

form

lyophilized (stabilized)

form

lyophilized

form

solution

packaging

pkg of 150 U (200 μl)

packaging

pkg of 150 U

packaging

pkg of 200 μg

packaging

pkg of 200 μL (150 U)

General description

Fluorescein is synthesized from m-dialkylaminophenols or resorcinols condensed with phthalic anhydrides under harsh conditions. Fab fragments bind to G proteins.
This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. (provided by Ref Seq)

Specificity

The polyclonal antibody reacts with free and bound fluorescein.

Application

Use Anti-Fluorescein-AP, Fab fragments for the detection of fluorescein-labeled compounds using:
  • Dot blot
  • ELISA
  • Immunohistocytochemistry
  • In situ hybridization
  • Southern blot
  • Western blot
  • Electrochemical biosensing technique for the detection of 16S rRNA

Biochem/physiol Actions

Anti-Fluorescein-AP, Fab fragments has been used in oligonucleotide capture, denaturation and detection (CDD).

Features and Benefits

Contents
Solution, stabilized

Preparation Note

  • Working concentration: The following concentrations depend on application and substrate and should be taken as a guideline. Dot blot: 150mU/ml
  • ELISA: 150 to 300mU/ml
  • Immunohistocytochemistry: 250 to 500mU/ml
  • In situ hybridization: 1.5 to 7.5U/ml
  • Southern blot: 150mU/ml
  • Western blot: 250 to 500 mU/ml

Working solution: 100mM Tris-HCl, 150mM NaCl, pH 7.5. If necessary 1% Blocking reagent (w/v), dry milk powder, 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available


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Megan Addison et al.
Developmental cell, 45(5), 606-620 (2018-05-08)
The patterning of tissues to form subdivisions with distinct and homogeneous regional identity is potentially disrupted by cell intermingling. Transplantation studies suggest that homogeneous segmental identity in the hindbrain is maintained by identity switching of cells that intermingle into another segment. We
Ana L Miranda-Angulo et al.
The Journal of comparative neurology, 522(4), 876-899 (2013-08-14)
The wall of the ventral third ventricle is composed of two distinct cell populations: tanycytes and ependymal cells. Tanycytes regulate many aspects of hypothalamic physiology, but little is known about the transcriptional network that regulates their development and function. We
Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores
Minta A, et al.
The Journal of Biological Chemistry, 264(14), 8171-8178 (1989)
Applications And Engineering Of Monoclonal Antibodies (1998)
Simplified Paper Format for Detecting HIV Drug Resistance in Clinical Specimens by Oligonucleotide Ligation
Panpradist N, et al.
PLoS ONE (2016)

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