MilliporeSigma
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11585550910

Roche

PCR DIG Labeling Mix

greener alternative

solution, suitable for PCR

Synonym(s):
nucleic acid labeling

form

solution

Quality Level

usage

sufficient for 2 x 25 assays (100 ul final reaction volume)

packaging

pkg of 500 μL (2 x 250 μl)

manufacturer/tradename

Roche

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

technique(s)

PCR: suitable

greener alternative category

storage temp.

−20°C

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This Item
112770659101117503391011277073910
PCR DIG Labeling Mix solution, suitable for PCR

Roche

11585550910

PCR DIG Labeling Mix

DIG DNA Labeling Mix

Roche

11277065910

DIG DNA Labeling Mix

DIG DNA Labeling Kit sufficient for 40 labeling reactions, kit of 1 (7 components), suitable for hybridization

Roche

11175033910

DIG DNA Labeling Kit

DIG RNA Labeling Mix sufficient for 20 reactions, solution

Roche

11277073910

DIG RNA Labeling Mix

packaging

pkg of 500 μL (2 x 250 μl)

packaging

pkg of 50 μL

packaging

kit of 1 (7 components)

packaging

pkg of 40 μL

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

usage

sufficient for 2 x 25 assays (100 ul final reaction volume)

usage

sufficient for 25 standard reactions

usage

sufficient for 40 labeling reactions

usage

sufficient for 20 reactions

General description

PCR DIG Labeling Mix is a nucleotide mixture that can be added directly to polymerase chain reactions (PCR) and the digoxigenin (DIG)-labeled nucleotide will be incorporated into the PCR product. Taq DNA polymerase, as well as Tth (Thermus thermophilus) DNA polymerase, can be used for the synthesis of DIG-labeled PCR products. The PCR DIG Labeling Mix can replace the unlabeled nucleotide mix in PCR. 10μl of the PCR DIG Labeling Mix is used in a standard 100μl PCR assay.
When using higher concentrations of DIG-deoxyuridinetriphosphate (dUTP) what is supplied with the PCR DIG Labeling Mix, the yield of the PCR product may be reduced, but however, the label intensity and the molecular weight of the PCR product is increased.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

The PCR DIG Labeling Mix is specifically designed for the sensitive detection of polymerase chain reaction (PCR) products and the sensitive analysis of PCR reactions. The PCR DIG Labeling Mix can also be used for the synthesis of hybridization probes. However, for the production of highly sensitive probes, for example, necessary when detecting single-copy genes on genomic blots, we recommend a PCR nucleotide mix with an increased concentration of DIG-dUTP (e.g., PCR DIG Probe Synthesis Kit).

PCR DIG Labeling Mix has been used in the preparation of digoxigenin-labeled riboprobes during the in vitro transcription step.

Quality

The PCR DIG Labeling Mix is function tested in PCR. Amplification products are assayed by dot blot and in hybridization experiments. DNases and RNases are not detectable according to the current Quality Control procedures.

Physical form

Solution, 10x concentrated: PCR DIG labeling mix is a mixture of the sodium salts of dATP, dCTP, dGTP, dTTP and digoxigenin-11-dUTP, lithium salt. The solution contains 2 mM dATP, dCTP, dGTP each, 1.9 mM dTTP, 0.1 mM digoxigenin-11-dUTP (DIG-11-dUTP) in 2x 250 μl water; pH 7.0.

Other Notes

For life science research only. Not for use in diagnostic procedures.

also commonly purchased with this product

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash


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Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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