11585550910

PCR DIG Labeling Mix

solution, suitable for PCR

• Synonym(s): nucleic acid labeling

Properties

• form: solution
• Quality Level: 100
• usage: sufficient for 2 x 25 assays (100 ul final reaction volume)
• packaging: pkg of 500 μL (2 x 250 μl)
• manufacturer/tradename: Roche
• greener alternative product characteristics: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• technique(s): PCR: suitable
• greener alternative category: Aligned
• storage temp.: −20°C

Description

General description

PCR DIG Labeling Mix is a nucleotide mixture that can be added directly to polymerase chain reactions (PCR) and the digoxigenin (DIG)-labeled nucleotide will be incorporated into the PCR product. Taq DNA polymerase, as well as Tth (Thermus thermophilus) DNA polymerase, can be used for the synthesis of DIG-labeled PCR products. The PCR DIG Labeling Mix can replace the unlabeled nucleotide mix in PCR. 10μl of the PCR DIG Labeling Mix is used in a standard 100μl PCR assay.
When using higher concentrations of DIG-deoxyuridinetriphosphate (dUTP) what is supplied with the PCR DIG Labeling Mix, the yield of the PCR product may be reduced, but however, the label intensity and the molecular weight of the PCR product is increased.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

The PCR DIG Labeling Mix is specifically designed for the sensitive detection of polymerase chain reaction (PCR) products and the sensitive analysis of PCR reactions. The PCR DIG Labeling Mix can also be used for the synthesis of hybridization probes. However, for the production of highly sensitive probes, for example, necessary when detecting single-copy genes on genomic blots, we recommend a PCR nucleotide mix with an increased concentration of DIG-dUTP (e.g., PCR DIG Probe Synthesis Kit).

PCR DIG Labeling Mix has been used in the preparation of digoxigenin-labeled riboprobes during the in vitro transcription step.

Quality

The PCR DIG Labeling Mix is function tested in PCR. Amplification products are assayed by dot blot and in hybridization experiments. DNases and RNases are not detectable according to the current Quality Control procedures.

Physical form

Solution, 10x concentrated: PCR DIG labeling mix is a mixture of the sodium salts of dATP, dCTP, dGTP, dTTP and digoxigenin-11-dUTP, lithium salt. The solution contains 2 mM dATP, dCTP, dGTP each, 1.9 mM dTTP, 0.1 mM digoxigenin-11-dUTP (DIG-11-dUTP) in 2x 250 μl water; pH 7.0.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): does not flash
• Flash Point(C): does not flash