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11796828001

Roche

High Pure PCR Template Preparation Kit

pkg of 100 purifications, suitable for DNA extraction

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Synonym(s):
DNA extraction

usage

sufficient for 100 purifications

Quality Level

manufacturer/tradename

Roche

packaging

pkg of 100 purifications

technique(s)

DNA extraction: suitable

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HPPCRPKRO1203367400105080576001
High Pure RNA Tissue Kit sufficient for 50 isolation(s), suitable for RT-PCR, suitable for Northern blotting

Roche

12033674001

High Pure RNA Tissue Kit

usage

sufficient for 100 purifications

usage

sufficient for 250 purifications (11732676001), sufficient for 50 purifications (11732668001)

usage

sufficient for 50 isolation(s)

usage

-

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

packaging

pkg of 100 purifications

packaging

pkg of 250 purifications (11732676001), pkg of 50 purifications (11732668001)

packaging

kit of for 50 isolations

packaging

kit of for 50 isolations

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

Low to medium throughput genomic DNA isolation.

High Pure PCR Template Preparation Kit; Instructions For Use

Nucleic acids bind to the surface of the glass fiber fleece in the presence of a chaotropic salt (guanidine HCl). This allows the High Pure filter tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants.

Note: A special Inhibitor Removal Buffer is included which allows the use of heparinized sample material (100 U/mL of heparin). This buffer increases the sensitivity and reproducibility of assays performed with the isolated nucleic acid, even when the sample contains heparin.

Capacity: The High Pure Spin Filter Tubes hold up to 700 μL sample volume.

Application

High Pure PCR Template Preparation Kit has been used in the extraction of genomic DNA from whole blood samples, cervical lesion specimens, gastric muscosal tissue and cervical carcinoma cell lines.
The High Pure PCR Template Preparation Kit purifies nucleic acids from a wide variety of sample materials including whole blood, cultured cells, and tissue. The isolated nucleic acids can be used for:
  • Long-template PCR
  • Real-time, quantitative PCR
  • SNP detection
  • Southern blotting
  • Cloning

Features and Benefits

The High Pure PCR Template Preparation Kit purifies nucleic acids from a wide variety of sample materials, including whole blood, cultured cells, and tissue samples. Bacteria and yeast require a specific pre-lysis treatment with lysozyme or lyticase.
  • Minimize DNA loss using a kit that removes contaminants without precipitation or other handling steps that degrade DNA.
  • Recover high molecular weight DNA (30 to 50 kb) that is suitable for long-template PCR.
  • Improve reliability and reproducibility in downstream applications (real-time, quantitative PCR).
  • Save time and maximize flexibility by preparing multiple PCR templates simultaneously.
  • Eliminate the use of hazardous organic compounds such as cesium chloride, phenol, chloroform, and ethidium bromide.

Components

  • Tissue Lysis Buffer
  • Binding Buffer
  • Proteinase K, recombinant PCR grade
  • Inhibitor Removal Buffer
  • Washing Buffer
  • Elution Buffer
  • High Pure Filter Tubes
  • Collection Tubes

Quality

DNA is isolated from 25 mg of calf thymus, 1 x 106 K562 cells, and 200 μl of EDTA whole blood. Yield is measured via OD for tissue and cell samples. The quality of the nucleic acid is controlled in an Expand Long Range PCR with a 9.3 kb amplification product for DNA derived from cells. PCR on a LightCycler® Instrument is performed on human blood research samples using kits for Factor V Leiden and CycA.

Principle

Blood cells or tissue are lysed by a short incubation with a special Lysis Buffer and Proteinase K in the presence of a chaotropic salt such as guanidine-HCl, which immediately inactivates all nucleases. Cellular nucleic acids bind selectively to special glass fibers pre-packed in the High Pure Purification Filter Tube. Bound nucleic acid is purified in a series of rapid wash-and-spin steps to remove contaminating cellular components. Finally, low salt elution releases the nucleic acid from the glass fiber. This simple method eliminates the need for organic solvent extractions and DNA precipitation, allowing for rapid purification of many samples simultaneously.

Legal Information

LightCycler is a registered trademark of Roche

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

does not flash

Flash Point(C)

does not flash


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