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COLLP-RO

Roche

Collagenase P

from Clostridium histolyticum

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Synonym(s):
collagenase
Enzyme Commission number:

biological source

Clostridium histolyticum

Quality Level

sterility

non-sterile

form

lyophilized

collagenase activity

>1.5 U/mg (Lyophilizate, Collagenase activity)

packaging

pkg of 1 g (11249002001)
pkg of 100 mg (11213857001)
pkg of 2.5 g (11213873001)
pkg of 500 mg (11213865001)

manufacturer/tradename

Roche

concentration

0.5-2.5 mg/mL

technique(s)

tissue processing: suitable

color

light brown to brown

solubility

water: soluble

NCBI accession no.

application(s)

life science and biopharma

foreign activity

Clostripain 24.099 U/mg
Protease 4.4 U/mg (Proteases)
Trypsin 53.428 U/mg (with BAEE)

storage temp.

2-8°C

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1 of 4

This Item
COLLH-ROCOLLD-ROCOLLB-RO
vibrant-m

COLLP-RO

Collagenase P

vibrant-m

COLLH-RO

Collagenase H

vibrant-m

COLLD-RO

Collagenase D

vibrant-m

COLLB-RO

Collagenase B

technique(s)

tissue processing: suitable

technique(s)

tissue processing: suitable

technique(s)

tissue processing: suitable

technique(s)

-

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

NCBI accession no.

WP_138210331

NCBI accession no.

WP_138210331

NCBI accession no.

WP_171012037

NCBI accession no.

-

application(s)

life science and biopharma

application(s)

life science and biopharma

application(s)

cell analysis

application(s)

sample preparation

color

light brown to brown

color

brown

color

light brown

color

-

General description

Collagenase P is an enzyme mixture prepared from the extracellular culture filtrate of a special Clostridium histolyticum strain.Collagenase P is a research biochemical, not manufactured under the US good manufacturing practices (GMP) regulations.
Note: Collagenase P is a non-sterile preparation of the culture supernatant of Clostridium histolyticum. It is neither designed nor intended for isolation of pancreatic islets for transplantation into humans and must not be used for such purposes.

Specificity

Enzyme activity:
Collagenase activity: >1.5U/mg (according to Wünsch) (+25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate)
Contaminating enzyme activities: trypsin, clostripain, and total proteolytic activity
Collagenase P has a very high collagenase activity (>1.5U/mg), and is function tested for the isolation of mouse pancreatic islets.
Specificity: Collagenase degrades native collagen. Clostripain, trypsin-like enzymes, and neutral proteases degrade other proteins as well.

Application

Collagenase from C. histolyticum is used for the dissociation of tissues for the establishment of primary cell cultures. Because of its high collagenase activity Collagenase P is particularly suitable for the isolation of pancreatic islets from mouse and rat (function tested). The preparation is also suitable for the isolation of adipocytes from epididymal fat pads of rats.

Features and Benefits

Contents:
Lyophilizate, nonsterile

Preparation Note

Activator: Ca2+
Inhibitors:
Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercaptoethanol
Collagenase is not inhibited by serum.
Clostripain inhibitors: TLCK
Trypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean)
Working concentration: 0.5 to 2.5mg/ml
0.5 to 1.5mg/ml for the isolation of pancreatic islets from mouse and rat pancreas.
Approximately 2mg/ml for the isolation of adipocytes.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable at -15 to -25°C. Prepare appropriate aliquots and avoid repeated freezing and thawing.

Reconstitution

Reconstitution in any balanced salt solution (e.g., HBSS)

Storage and Stability

Store dry

Other Notes

For life science research only. Not for use in diagnostic procedures.

pictograms

Exclamation markHealth hazard

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


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Collagenase Guide.Collagenases, enzymes that break down the native collagen that holds animal tissues together, are made by a variety of microorganisms and by many different animal cells.

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