MilliporeSigma
All Photos(1)

RPOLSP6-RO

Roche

SP6 RNA Polymerase

from Escherichia coli BL 21/pSR3

Sign Into View Organizational & Contract Pricing

Select a Size

Synonym(s):
mRNA, polymerase

biological source

Escherichia coli (BL 21/pSR3)

Quality Level

form

solution

packaging

pkg of 1,000 U (10810274001)
pkg of 5,000 U (11487671001)

manufacturer/tradename

Roche

storage temp.

−20°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
RPOLT7-RORPOLT3-ROTAQN-RO
SP6 RNA Polymerase from Escherichia coli BL 21/pSR3

Roche

RPOLSP6-RO

SP6 RNA Polymerase

T7 RNA Polymerase from Escherichia coli BL 21/pAR 1219

Roche

RPOLT7-RO

T7 RNA Polymerase

T3 RNA Polymerase from Escherichia coli HB101

Roche

RPOLT3-RO

T3 RNA Polymerase

Taq DNA Polymerase, dNTPack suitable for PCR, optimum pH ~9.0 (20 °C), dNTPs included

Roche

TAQN-RO

Taq DNA Polymerase, dNTPack

form

solution

form

solution

form

solution

form

liquid

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

packaging

pkg of 1,000 U (10810274001)

packaging

pkg of 1,000 U (10881767001), pkg of 5,000 U (10881775001)

packaging

pkg of 1,000 U (11031163001), pkg of 5,000 U (11031171001)

packaging

pkg of 1,000 U (04728882001 [4 x 250 U]), pkg of 2,500 U (04728904001 [10 x 250 U]), pkg of 5,000 U (04728858001 [20 x 250 U]), pkg of 100 U (04728866001), pkg of 500 U (04728874001 [2 x 250 U])

General description

With this system, homogeneously labeled single-stranded RNA can be generated. Transcripts can be non-radioactively labeled with biotin or DIG-11-UTP or radioactively labeled to high specific activity with [α-32P] or [α-35S] labeled nucleotides.

Contents:
  • SP6 RNA Polymerase, ≥20 U/μl, in buffer, pH 7.9
  • Transcription Buffer, 10x concentrated

Specificity

Promoter specifity

SP6 RNA polymerase is extremely promoter-specific and only transcribes bacteriophage SP6 DNA or DNA cloned downstream of a SP6 promoter (e.g., pSPTBM20; pSPTBM21).

Heat inactivation: Stop the reaction by adding 2 μl 0.2 M EDTA (pH 8.0) and/or heat to 65 °C.

Application

SP6 RNA Polymerase can transcribe RNA from cloned DNA templates that are downstream from an SP6 promoter. The synthesis can be performed with labeled NTPs to generate highly labeled RNA. Synthesized RNA can be used in many applications, including:
  • RNA or DNA blotting techniques
  • In situ hybridization
  • RNase protection studies: Transcripts synthesized by the enzyme are used as precursor RNA for studies on RNA splicing and processing.
  • Synthesis of capped RNA in vitro with addition of m7GpppG or m7GpppA in excess over GTP or ATP during the transcription reaction. The generated antisense RNA can be introduced into cells to suppress the expression of the corresponding genes.
  • The synthesis of antisense RNA probes

Quality

Test Buffer
40 mM Tris-HCl, pH 8.0 (+20°C), 6 mM MgCl2, 10 mM dithiothreitol, 2 mM spermidine, pH approximately 8.0 (+20°C).
Absence of Endonucleases
1. 1 μg lambda DNA is incubated with SP6 RNA polymerase for 4 hours at +37°C in 25 μl test buffer. The number of enzyme units which show no degradation of lambda DNA is > 30 U.
2. 1 μg Eco RI/Hind III fragments of lambda DNA is incubated with SP6 RNA polymerase for 4 hours at +37°C in 25 μl test buffer. The number of enzyme units which show no alteration of the banding pattern is > 30 U.
Absence of Nicking Activity
1 μg pBR322 DNA is incubated with SP6 RNA polymerase for 4 hours at +37°C in 25 μl test buffer. The number of enzyme units which show no relaxing of supercoiled structure is > 30.
Absence of RNases
4 μg MS2 RNA are incubated with SP6 RNA polymerase for 4 hours at +37°C in 50 μl test buffer. The number of enzyme units which show no degradation of MS2 RNA is > 30.
Performance in Transcription Assay
SP6 RNA polymerase is function tested in the SP6/T7 Transcription Kit (Cat. No. 10 999 644 001). The incorporation rate in the standard assay with 0.5 μg pST18 neo DNA linearized with Eco RI and 50 mCi [alpha-32P] CTP, [400 Ci/mmol (15 TBq/mmol)] gives >50% of the input radioactivity in 20 minutes.

Specifications

Synthesis of hybridization probes: SP6 RNA polymerase allows highly efficient production of homogeneously labeled RNA. This labeled RNA may be used as hybridization probes in Southern, northern, and dot blots, as well as in situ hybridizations.
Suitable labels: Transcripts can be nonradioactively labeled with biotin-16-UTP, DIG-11-UTP, or fluorescein-12-UTP. They may also be radioactively labeled to high specific activity with [a-32P]- or [a-35S]-labeled nucleotides.

Note: Roche has 10x concentrated RNA Labeling Mixes that are specially designed for DIG-, biotin-, and fluorescein-labeling. These mixes work well with SP6 RNA Polymerase.

Unit Definition

One unit is the enzyme activity which incorporates 1 nmol CMP in acid-precipitable RNA products within 60 minutes at +37 °C.

Volume Activity: ≥20 U/μl

Preparation Note

Activator: BSA/spermine

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description

  • SP6 RNA Polymerase, in buffer, pH 7.9 ≥20 U/μl

  • Transcription Buffer 10x concentrated

also commonly purchased with this product

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 6

1 of 6

Digoxigenin-11-UTP

Roche

DIGUTP-RO

Digoxigenin-11-UTP

BM-Purple Roche, pkg of 100 mL, solution

Roche

11442074001

BM-Purple

DIG DNA Labeling Mix

Roche

11277065910

DIG DNA Labeling Mix

PD-166866 ≥98% (HPLC)

Sigma-Aldrich

PZ0114

PD-166866

NBT/BCIP Stock Solution

Roche

11681451001

NBT/BCIP Stock Solution

Ezgi Hacisuleyman et al.
Nature structural & molecular biology, 21(2), 198-206 (2014-01-28)
RNA, including long noncoding RNA (lncRNA), is known to be an abundant and important structural component of the nuclear matrix. However, the molecular identities, functional roles and localization dynamics of lncRNAs that influence nuclear architecture remain poorly understood. Here, we
Adam D Langenbacher et al.
EvoDevo, 7, 9-9 (2016-04-14)
Germ cells are specified during early development and are responsible for generating gametes in the adult. After germ cells are specified, they typically migrate to a particular niche in the organism where they reside for the remainder of its lifetime.
Camilo Riquelme-Guzmán et al.
eLife, 11 (2022-10-12)
Early events during axolotl limb regeneration include an immune response and the formation of a wound epithelium. These events are linked to a clearance of damaged tissue prior to blastema formation and regeneration of the missing structures. Here, we report
Sonja Oberland et al.
Frontiers in cellular neuroscience, 9, 366-366 (2015-10-07)
Olfactory signals influence food intake in a variety of species. To maximize the chances of finding a source of calories, an animal's preference for fatty foods and triglycerides already becomes apparent during olfactory food search behavior. However, the molecular identity
Adam D Langenbacher et al.
Genesis (New York, N.Y. : 2000), 53(1), 194-201 (2014-09-03)
Botryllus schlosseri is a colonial ascidian with characteristics that make it an attractive model for studying immunology, stem cell biology, evolutionary biology, and regeneration. Transcriptome sequencing and the recent completion of a draft genome sequence for B. schlosseri have revealed

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service