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03677

Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt dihydrate

BioUltra, ≥99.0% (T)

Synonym(s):

Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na2, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na2

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About This Item

Empirical Formula (Hill Notation):
C10H14N2Na2O8 · 2H2O
CAS Number:
6381-92-6
Molecular Weight:
372.24
Beilstein/REAXYS Number:
3900609
EC Number:
205-358-3
UNSPSC Code:
12352107
PubChem Substance ID:
24845544
NACRES:
NA.25
assay:
≥99.0% (T)
grade:
Molecular Biology
form:
powder or crystals
solubility:
H2O: 0.1 M at 20 °C, clear, colorless

grade

Molecular Biology

Quality Level

200

product line

BioUltra

assay

≥99.0% (T)

form

powder or crystals

reaction suitability

reagent type: chelator

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

pH

4.0-5.5 (25 °C, 0.1 M in H2O)

mp

248 °C (dec.) (lit.)

solubility

H2O: 0.1 M at 20 °C, clear, colorless

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Application

Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.
Ethylenediaminetetraacetic acid disodium salt dehydrate can be used to study molecular biology, biochemicals, solutions and reagents, biological buffers, DNA and RNA purification, inorganic salts, essential chemicals and chelators. Ethylenediaminetetraacetic acid disodium salt dehydrate has been used in a study to advance monitoring of illegal thyreostatic drugs in livestock. Ethylenediaminetetraacetic acid disodium salt dehydrate has also been used in a study for the determination of 232Th in seawater by ICP-MS after preconcentration and separation using a chelating resin.

Other Notes

Running-buffer component for non-denaturing or urea-containing denaturing polyacrylamide gels in nucleic acid electrophoresis

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

hcodes

H332,H373

Hazard Classifications

Acute Tox. 4 Inhalation - STOT RE 2 Inhalation

target_organs

Respiratory Tract

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM0132
BCCJ4740
BCCJ4742
BCCH7532
BCCF9021

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R.C. Ogden et al.
Methods in Enzymology, 152, 65-65 (1987)
J Vanden Bussche et al.
Analytical and bioanalytical chemistry, 403(10), 2973-2982 (2012-02-22)
Thyreostatic drugs, illegally administrated to livestock for fattening purposes, are banned in the European Union since 1981. For monitoring their illegal use, sensitive and specific analytical methods are required. In this context, the knowledge of the stability in a matrix
Suli Huang et al.
PloS one, 10(2), e0117007-e0117007 (2015-02-07)
microRNA (miRNA) plays a role in the pathogenesis of ischemic stroke, and single nucleotide polymorphisms in miRNA genes may contribute to disease susceptibility. However, the effect of miR-146a, miR-196a2, and miR-499 polymorphisms on ischemic stroke susceptibility has been rarely reported.
G Lima-Oliveira et al.
British journal of biomedical science, 70(1), 6-9 (2013-04-27)
This study assesses the use of different dry K2 (dipotassium) EDTA vacuum tubes and whether or not they might represent a bias in haematological testing. Blood was collected in three dipotassium EDTA vacuum tubes from different manufacturers: Venosafe, Vacuette and
Lisa M Ryno et al.
Biomacromolecules, 15(8), 2944-2951 (2014-07-18)
End-functionalized macromolecular starch reagents, prepared by reductive amination, were grafted onto a urethane-linked polyester-based backbone using copper-catalyzed azide-alkyne cycloaddition (CuAAC) chemistry to produce novel amphiphilic hybrid graft copolymers. These copolymers represent the first examples of materials where the pendant chains
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