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114367

Sigma-Aldrich

Bromocresol Green sodium salt

90% (HPLC), ACS reagent, Dye content 90 %

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Synonym(s):
3′,3″,5′,5″-Tetrabromo-m-cresolsulfonphthalein sodium salt
Empirical Formula (Hill Notation):
C21H13Br4NaO5S
CAS Number:
Molecular Weight:
720.00
EC Number:
MDL number:
PubChem Substance ID:

grade

ACS reagent

Quality Level

agency

suitable for SM 2310
suitable for SM 2320

assay

90% (HPLC)

form

powder or crystals

composition

Dye content, 90%

visual transition interval

3.8, yellow(Visual Interval Pass)
3.8-5.2, yellow to blue
4.5, green
5.4, blue(Visual Interval Pass)

mp

230 °C (dec.) (lit.)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].Cc1c(Br)c(O)c(Br)cc1\C(=C2\C=C(Br)C(=O)C(Br)=C2C)c3ccccc3S([O-])(=O)=O

InChI

1S/C21H14Br4O5S.Na/c1-9-12(7-14(22)19(26)17(9)24)21(13-8-15(23)20(27)18(25)10(13)2)11-5-3-4-6-16(11)31(28,29)30-21;/h3-8,26-27H,1-2H3;/q;+1/p-1

InChI key

HEFSGAHJDGZCHA-UHFFFAOYSA-M

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This Item
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Bromocresol Green sodium salt 90% (HPLC), ACS reagent, Dye content 90 %

114367

Bromocresol Green sodium salt

Bromocresol Green sodium salt crystalline

B1256

Bromocresol Green sodium salt

Bromocresol Purple sodium salt indicator grade, Dye content 90 %

860891

Bromocresol Purple sodium salt

Bromocresol Green Sultone Form for microscopy (Bot., Hist., Vit.), indicator (pH 3.8-5.4)

17470

Bromocresol Green Sultone Form

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

form

powder or crystals

form

crystalline

form

powder

form

powder

composition

Dye content, 90%

composition

-

composition

Dye content, 90%

composition

-

assay

90% (HPLC)

assay

-

assay

-

assay

-

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

General description

Bromocresol Green (BCG), also known as bromcresol green, is a synthetic, anionic, triphenylmethane dye belonging to the phthalein and sulphonphthalein dyes group. It is commonly used as a pH indicator as it changes color from yellow to blue-green at pH 3.8-5.4. The monosodium salt of bromocresol green is highly ionizable in water. The dye at a higher pH level produces a large and weakly lipophilic dianion.

Application

Bromocresol green serves as a pH indicator in biology and medicinal experiments.
  • It is used widely as a vital stain to study blood-brain permeability.
  • It is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations, and in charge-transfer complexation processes.
  • It is used in TLC for the visualization of compounds with functional groups whose pKa is below 5.0.
  • It has been used in the pH-sensitive colorimetric determination of glutamate decarboxylase activity.
  • It has also been employed in the evaluation of paper-analytical devices for colorimetric analysis of serum samples.
  • It is suitable as a selective inhibitor in kidney physiology studies.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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