69964

MRS Agar

NutriSelect^® Basic, powder, nonselective for Lactobacillus Spp., pack of 500 g

• Synonym(s): Lactobacillus Agar according to DeMan, Rogosa and Sharpe, Lactobacillus Agar according to DeMan, Rogosa, and Sharpe
• NACRES: NA.74
• UNSPSC Code: 41171605

Properties

• Product Name: MRS Agar, NutriSelect^® Basic, powder, nonselective for Lactobacillus Spp., pack of 500 g
• Quality Level: 100
• sterility: non-sterile
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 12 g/L ; dipotassium hydrogen phosphate, 2 g/L ; D(+)-glucose, 20 g/L ; magnesium sulfate (equivalent to 0.2 g/lheptahydrate), 0.1 g/L ; manganous sulfate monhydrate, 0.05 g/L ; meat extract, 5 g/L ; sodium acetate, 5 g/L ; triammonium citrate, 2 g/L ; universal peptone, 10 g/L ; yeast extract, 5 g/L
• packaging: pack of 500 g
• manufacturer/tradename: NutriSelect^® Basic
• technique(s): microbiological culture: suitable
• final pH: 6.5±0.2 (25 °C)
• application(s): agriculture; bioburden testing; environmental; food and beverages; microbiology
• suitability: nonselective for Lactobacillus Spp.; selective by low pH for Bifidobacterium spp.

Description

Other Notes

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Preparation Note

Suspend 61.15 g in 1000 ml distilled water containing 1 ml polysorbate 80 (TWEEN^® 80; Sigma P8074). Boil to dissolve the medium completely and mix thoroughly. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If necessary adjust the pH with glacial acetic acid after sterilization.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Application

MRS Agar is widely used for the detection, isolation, and cultivation of Lactobacillus spp. and other lactic acid bacteria from food, beverages, and environmental samples. It is applied in agriculture, food and beverage quality control, environmental monitoring, and microbiology research. The medium supports the growth of fastidious Lactobacillus species, such as Lactobacillus brevis and Lactobacillus fermentum, and is often used in CO₂-enriched atmospheres to enhance growth.

Features and Benefits

NutriSelect^® Basic is a powdered culture media offering

• Quality control with growth promotion test according to industry standards
• Quality assurance acc. to ISO 9001
• Cost-efficient powdered media

General description

MRS Agar, also known as DeMan-Rogosa-Sharpe Agar, is a microbiological medium specifically designed for the enrichment, cultivation, and isolation of Lactobacillus species from various materials. It is a non-selective medium with a low pH that favors the growth of Lactobacillus spp. and Bifidobacterium spp. The medium contains essential nutrients such as glucose, meat extract, yeast extract, and peptones, which provide nitrogenous and carbonaceous compounds, amino acids, and vitamins necessary for microbial growth. Sodium acetate and ammonium citrate enhance selectivity by inhibiting the growth of unwanted organisms. The media contains polysorbate (Tween 80), acetate, magnesium, and manganese, which are known to act as specific growth factors for Lactobacilli, and provide a rich nutrient base.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)