MilliporeSigma
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71019BC

Millipore

GDS MPX ID for Top STEC

BioControl, Molecular based secondary screening PCR test for the detection and identification of "Top Six" non-O157 Shiga Toxigenic E. coli (STEC) in food and environmental samples

Agency

AOAC® (PTM)

Quality Level

shelf life

6‑12 mo.

packaging

pkg of 52 tests

manufacturer/tradename

Assurance GDS®
BioControl Systems 71019-52

technique(s)

PCR: suitable

application(s)

food and beverages
pathogen testing

compatibility

for use with GDS Rotor-Gene

storage temp.

2-8°C

suitability

enterovirulent E. coli (EEC) (shiga toxigenic)

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71037BC71015BC71018BC
GDS MPX ID for Top STEC BioControl, Molecular based secondary screening PCR test for the detection and identification of "Top Six" non-O157 Shiga Toxigenic E. coli (STEC) in food and environmental samples

Millipore

71019BC

GDS MPX ID for Top STEC

GDS MPX Top 7 STEC BioControl, Molecular based PCR test for the detection of E. coli O157:H7 and the "Top Six" non-O157 Shiga Toxigenic E. coli (STEC) in food

Millipore

71015BC

GDS MPX Top 7 STEC

shelf life

6‑12 mo.

shelf life

6‑12 mo.

shelf life

6‑12 mo.

shelf life

6‑12 mo.

packaging

pkg of 52 tests

packaging

-

packaging

pkg of 100 tests

packaging

pkg of 100 tests

manufacturer/tradename

Assurance GDS®, BioControl Systems 71019-52

manufacturer/tradename

-

manufacturer/tradename

Assurance GDS®, BioControl Systems 71015-100, BioControl Systems 71015-576

manufacturer/tradename

Assurance GDS®, BioControl Systems 71018-100

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

application(s)

food and beverages
pathogen testing

application(s)

food and beverages
pathogen testing

application(s)

food and beverages
pathogen testing

application(s)

food and beverages
pathogen testing

General description

Assurance® GDS is an advanced genetic detection system for food pathogens. The MPX ID for Top STEC is a secondary automated nucleic acid amplification system for the identification of “Top Six” non-O157 Shiga Toxigenic E. coli (STEC). The Top 6 non-O157 STEC are defined as E. coli belonging to serogroups O103, O111, O121, O145, O26, or O45 that possess both the eae gene and at least one of the Shiga Toxin genes (stx1 or stx2). GDS MPX ID utilizes a proprietary PickPen® IMS-based sample preparation procedure to quickly and effectively capture organisms belonging to the 6 specific Top STEC O-serogroups prior to genetic analysis for the associated pathogenicity genes. Each of the 3 unique eae gene sequences targeted by MPX ID are highly correlated plus one or more of the 6 O-serogroups comprising Top 6 non-O157 STEC. Because there are 3 different eae gene targets and 6 different O-serogroup targets, the PCR reactions have been divided between 2 Amplification Tubes. Each tube contains an internal control and all necessary PCR reagents. Results will automatically be combined between the 2 tubes via GDS Rotor-Gene® software.

Application

Assurance® GDS MPX ID for Top STEC is designed to follow GDS MPX Top 7 STEC (PN 71015BC) for the identification of “Top Six” non-O157 Shiga Toxigenic E. coli (STEC) in a variety of foods, ingredients, and environmental samples. Additionally, GDS MPX ID can be used to confirm isolated colonies from samples presumptive positive for Top 6 STEC.

Features and Benefits

  • Designed specifically to follow GDS MPX Top STEC assays
  • Identification of the specific O serogroup(s) responsible for the positive STEC result and verifies that all targets relevant to the definition of a Top 6 STEC (eae, stx and O group) are present within a single organism
  • Confirm isolated colonies from samples presumptive positive for Top 6 STEC
  • Utilizes the same single-step mEHEC® enrichment media common to all BioControl methods for Salmonella, E.coli O157:H7, Shiga Toxin genes, and Top STEC
  • Only 14 hours from start to finish

Components

  • MPX Group 1 STEC Amplification Tubes
  • MPX Group 2 STEC Amplification Tubes
  • Top 6 STEC Concentration Reagent
  • Resuspension Buffer Tq
  • Top STEC Wash Solution

Compatibility

BioControl GDS

Testing Method

PCR, Immunomagnetic Separation (IMS)

Legal Information

AOAC is a registered trademark of AOAC International
Assurance is a registered trademark of BioControl Systems, Inc.
Assurance GDS is a registered trademark of BioControl Systems, Inc.
PICKPEN is a registered trademark of BioControl Systems, Inc.
Rotor-Gene is a registered trademark of Qiagen GmbH
mEHEC is a registered trademark of BioControl Systems, Inc.

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3


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Articles

Molecular Detection for Rapid Food Pathogen Testing and Identification

Assurance® GDS is an integrated solution for food pathogen detection. It offers faster pathogen testing results with high accuracy to meet the demanding needs of food, environmental and cannabis microbiology testing challenges.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service