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Sigma-Aldrich

Basic Fuchsin

certified by the Biological Stain Commission, Dye content ≥88 %

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Synonym(s):
Basic Parafuchsin, Basic Red 9, Magenta O, Parafuchsin hydrochloride, Paramagenta hydrochloride, Pararosaniline chloride, Pararosaniline hydrochloride
Empirical Formula (Hill Notation):
C19H17N3 · HCl
CAS Number:
Molecular Weight:
323.82
Colour Index Number:
42500
Beilstein/REAXYS Number:
4164603
EC Number:
MDL number:
PubChem Substance ID:

grade

certified by the Biological Stain Commission

Quality Level

form

powder

composition

Dye content, ≥88%

color

green to dark green

pH

1.0-3.1, purple to red

mp

268-270 °C (dec.) (lit.)

λmax

544 nm

ε (extinction coefficient)

≥11000 at 235-239 nm in 50% ethanol at 0.003 g/L
≥17000 at 287-291 nm in 50% ethanol at 0.003 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Nc1ccc(cc1)C(\c2ccc(N)cc2)=C3/C=CC(=[NH2+])C=C3

InChI

1S/C19H17N3.ClH/c20-16-7-1-13(2-8-16)19(14-3-9-17(21)10-4-14)15-5-11-18(22)12-6-15;/h1-12,20H,21-22H2;1H

InChI key

JUQPZRLQQYSMEQ-UHFFFAOYSA-N

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Application

Basic fuchsin has been used as a lignin-detecting fluorogenic dye for fluorescence-based imaging of lignin in the basal internodes of three Brachypodium distachyon ecotypes.

Biochem/physiol Actions

Basic fuchsin belongs to the triphenylmethane group. Combining basic fuchsin with sulfurous acid results in the formation of fuchsin sulfurous acid, also referred to as Feulgen reagent or Schiff′s reagent. This reagent is responsible for the staining of chromosomal DNA. Basic fuchsin is also used for the staining of lignin in plant tissue samples.

Suitability

Certified for use for acid-fast staining with carbol-fuchsin, as ENDO medium, and as Schiff′s reagent in the Periodic Acid Schiff (PAS) and Feulgen techniques.

Legal Information

Magenta is a trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Carc. 1B

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges


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