I0765
Immersion oil
viscosity 150 cSt (lit.)
Synonym(s):
immersion medium for light microscopy
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About This Item
UNSPSC Code:
12171500
NACRES:
NA.47
Recommended Products
biological source
synthetic
Quality Level
color
yellow
viscosity
150 cSt(lit.)
density
0.9 g/cm3 at 23 °C
application(s)
hematology
histology
storage temp.
room temp
General description
Immersion Oil has a defined refractive index of about 1.516. It is important that the refractive index (nD) is about 1.5, similar to that of glass. This enables a homogeneous oil immersion to be achieved. Immersion media are used in conjunction with immersion objectives and are located between the surface of the specimen and the lens of the microscope. Immersion oils practically eliminate light beam deflection so that the effectiveness of the lens is considerably increased.
Application
Immersion oil has been used in the following applications:
- in epifluorescent microscopy and cell identification
- in the assembly and filling of the microinjecting system
- in microscopy to reliably visualize dendritic spines
Biochem/physiol Actions
Immersion oil has a larger impact on 3D resolution. Specifically, matching the immersion oil refractive index to glass, reliably enhances the ability to visualize dendritic spines.
signalword
Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Asp. Tox. 1 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
159.8 °F
flash_point_c
71 °C
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A simple pressure microinjecting system for delivery of small substance volumes to the brain: application to the developmental study of thalamo-cortical projections in foetal and neonatal rats.
Sbriccoli A, et al.
Brain Res. Protoc., 3(3), 257-263 (1999)
Impact of immersion oils and mounting media on the confocal imaging of dendritic spines.
Peterson BM, et al.
Journal of Neuroscience Methods, 242, 106-111 (2015)
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