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Nicotine metabolites in human urine (frozen)

NIST® SRM® 3671

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certified reference material

Quality Level


pkg of 3 x 10 mL




GC/MS: suitable
LC/MS: suitable


clinical research


matrix material

General description

A unit of Nicotine metabolites in human urine (SRM 3671) consists of three vials, each containing 10 mL of frozen human urine, one bottle of each of three levels. This standard reference material (SRM) was prepared from normal human urine collected from three different populations: nonsmokers without environmental exposure to tobacco smoke, nonsmokers with exposure to “secondhand” smoke, and smokers who smoke at least one pack of cigarettes per day.

SRM 3671_cert

SRM 3671 _SDS


Nicotine metabolites in human urine standard reference material (SRM) is intended primarily for use in evaluating the accuracy of procedures for the determination of nicotine metabolites in human urine. It is also intended for use in validating working or secondary reference materials.

Preparation Note

  • The urine is shipped frozen (on dry ice) and, upon receipt, should be stored frozen until ready for use.
  • Bottles of the SRM to be analyzed should be removed from the freezer and thawed to room temperature (20 °C to 25 °C).
  • After the material is thawed to room temperature, it should be used immediately.
  • The material should be swirled gently to mix before aliquots are withdrawn.

Other Notes

  • SRM 3671 is intended for research use only.
  • Details on expiration, storage, safety, usage, and source are provided in the NIST certificate
  • Information on biomaterials, disposal, and transport is available in the SDS

Legal Information

NIST is a registered trademark of National Institute of Standards and Technology
SRM is a registered trademark of National Institute of Standards and Technology

Storage Class

12 - Non Combustible Liquids




Not applicable


Not applicable

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Arezoo Habibagahi et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1162, 122490-122490 (2020-12-29)
Tobacco use, of which cigarette smoking is the most common, is a global health concern and is directly linked to over 7 million premature deaths annually. Measurement of the levels of tobacco-related biomarkers in biological matrices reflects human exposure to

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