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NIST8375

Microbial Genomic DNA Standards for Sequencing Performance Assessment

(MG-001, MG-002, MG-003, MG-004)

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grade

certified reference material

manufacturer/tradename

NIST®

technique(s)

electrophoresis: suitable

application(s)

genomic analysis

format

multi-component solution

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NIST8392NIST2373MBD0012
manufacturer/tradename

NIST®

manufacturer/tradename

NIST®

manufacturer/tradename

NIST®

manufacturer/tradename

-

grade

certified reference material

grade

-

grade

-

grade

-

format

multi-component solution

format

-

format

-

format

-

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

PCR: suitable, qPCR: suitable

technique(s)

DNA extraction: suitable, DNA sequencing: suitable, PCR: suitable

General description

This microbial genomic DNA standard is a reference material (RM) that consists of four vials. The genomic DNA is extracted from cell cultures. Each vial contains approximately 2 µg of microbial genomic DNA in TE buffer (10 mM TRIS, 1 mM EDTA, pH 8.0).

  • MG-001 Salmonella Typhimurium LT2
  • MG-002 Staphylococcus aureus
  • MG-003 Pseudomonas aeruginosa
  • MG-004 Clostridium sporogenes
NIST8375_Cert

NIST8375_SDS

Application

This reference material (RM) is suitable for validation, optimization, process evaluation, and performance assessment of microbial whole genome sequencing.

Features and Benefits

  • Enables the assessment of the performance of high-throughput microbial DNA sequencing methods.
  • It does not assess pre-analytical steps such as DNA extraction as the RM is extracted DNA.
  • Challenges sequencing library preparation, sequencing machines, base calling algorithms, and subsequent bioinformatics analyses.
  • This genomic DNA is extracted from cell cultures.

Other Notes

  • Refer to the SDS for additional information on biomaterials, disposal, and transport.
  • Details on expiration, maintenance, usage, storage, preparation, stability, homogeneity, and purity are available in the certificate.

Legal Information

NIST is a registered trademark of National Institute of Standards and Technology
SRM is a registered trademark of National Institute of Standards and Technology

Storage Class

10 - Combustible liquids


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