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NIST972A

Vitamin D metabolites in frozen human serum

NIST® SRM® 972a

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NACRES:
NA.24

grade

certified reference material

Quality Level

form

liquid

packaging

pkg of 4 x 1 mL

manufacturer/tradename

NIST®

technique(s)

mass spectrometry (MS): suitable

application(s)

clinical testing

format

matrix material

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This Item
NIST2969NIST2970NIST1951C
Lipids in frozen human serum NIST® SRM® 1951c

NIST1951C

Lipids in frozen human serum

form

liquid

form

-

form

-

form

liquid

manufacturer/tradename

NIST®

manufacturer/tradename

NIST®

manufacturer/tradename

NIST®

manufacturer/tradename

NIST®

application(s)

clinical testing

application(s)

clinical testing

application(s)

clinical testing

application(s)

clinical testing

format

matrix material

format

multi-component solution

format

multi-component solution

format

matrix material

packaging

pkg of 4 x 1 mL

packaging

pkg of 2 × 1 mL

packaging

pkg of 2 × 1 mL

packaging

pkg of 4 vials (2 ea conc)

General description

Determining total 25-hydroxy vitamin D [25(OH)D] concentration in serum, the sum of 25-hydroxyvitamin D2 [25(OH)D2] and 25-hydroxyvitamin D3 [25(OH)D3] is generally considered a reliable indicator of vitamin D status. Measurement of 24R, 25(OH)2D3 in serum is considered a catabolism marker and an indicator of kidney disease. A unit of SRM 972a contains 1 mL of four vials (levels 1 through 4) of frozen serum with different concentration levels of 25-hydroxyvitamin D [25(OH)D] and 24R, 25-dihydroxy vitamin D3 [24R,25(OH)2D3].

NIST972A_Cert

NIST972A_SDS

Application

This vitamin D metabolite standard reference material (SRM) is intended for use as an accuracy control in the evaluation of methods for determining the amount-of-substance concentration of vitamin D metabolites in human serum. This SRM can also be used as a quality assurance tool for assigning values to in-house control materials for these constituents.
Vitamin D metabolites in frozen human serum has been used as a reference material for the calibration and standardization of 25-hydroxyvitamin D (25(OH)D) from dried blood spot samples by liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Biochem/physiol Actions

Vitamin D known as cholecalciferol exists as vitamin D3. It is taken in the diet through fortified dairy products and fish oils. It is synthesized in the skin from 7-dehydrocholesterol by ultraviolet irradiation. The biologically active form of vitamin D, 25(OH)2D3, is essential for mineral metabolism, and for other physiological functions like inhibition of growth of cancer cells and protection against certain immune mediated disorders.

Other Notes

  • Information on expiry, storage, and usage is provided in the NIST certificate.
  • Notes on Biomaterials, disposal, and transport are available on NIST MSDS.

Preparation Note

  • The vials should be stored in the dark at a temperature between –20 °C and –80 °C.
  • Before use, the vial/s must be thawed at room temperature for about 30 mins in subdued light.

Legal Information

NIST is a registered trademark of National Institute of Standards and Technology
SRM is a registered trademark of National Institute of Standards and Technology

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Silvia Giuliani et al.
European journal of nutrition, 58(6), 2511-2520 (2018-08-09)
Developed countries have a high prevalence of vitamin D deficiency. In previous studies, 25(OH)D was predominantly measured by immunoassays. The present study assessed serum 25(OH)D in a very large Southern European outpatient cohort by liquid chromatography tandem mass spectrometry (LC-MS/MS).
A Mena-Bravo et al.
Journal of chromatography. A, 1451, 50-57 (2016-05-18)
A method based on automated on-line solid phase extraction coupled to two-dimensional liquid chromatography with tandem mass spectrometry detection (SPE-2DLC-MS/MS) is here reported for vitamin D metabolite profiling in human serum with absolute quantitation. Two-dimensional LC was configured with two
Carl Jenkinson et al.
Clinical chemistry and laboratory medicine, 59(10), 1642-1652 (2021-05-21)
Clinical evaluation of vitamin D status is conventionally performed by measuring serum levels of a single vitamin D metabolite, 25-hydroxyvitamin D predominantly by immunoassay methodology. However, this neglects the complex metabolic pathways involved in vitamin D bioactivity, including two canonical
Overestimation of 25-hydroxyvitamin D3 by increased ionisation efficiency of 3-epi-25-hydroxyvitamin D3 in LC-MS/MS methods not separating both metabolites as determined by an LC-MS/MS method for separate quantification of 25-hydroxyvitamin D3, 3-epi-25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 in human serum
van den Ouweland JMW, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 967, 195-202 (2014)
Simplified 25-hydroxyvitamin D standardization and optimization in dried blood spots by LC-MS/MS
Makowski AJ, et al.
Journal of AOAC (Association of Official Analytical Chemists) International, 100(5), 1328-1336 (2017)

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