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Bacillus spizizenii WDCM 00003 Vitroids

80-130 CFU mean value range, certified reference material, suitable for microbiology, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

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Bacillus subtilis subsp. spizizenii WDCM 00003 Vitroids, Certified reference material, Microbiological standards,, Bacillus subtilis subsp. spizizenii WDCM 00003 Vitroids


certified reference material

Quality Level


ISO 17034
ISO/IEC 17025


solid ()


certificate is enclosed in each package

shelf life

limited shelf life, expiry date on the label


package of 10 discs


Manufactured by: Sigma-Aldrich Production GmbH, Switzerland



storage temp.



Bacillus spizizenii
Bacillus spp.

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General description

Bacillus spizizenii WDCM 00003 Vitroids are Certified Microorganisms Standards (Certified Microbiological Reference Materials), which can be used as quantitative and qualitative control organisms. They contain colony-forming units (CFUs) in the range of 80-130 CFU per disc.

The technology allows long-term conservation and renders the organisms resistant to temperature changes. They are intended to be used as daily internal quality control organisms.

These microorganisms are certified according to ISO/IEC 17025 and produced under reproducible conditions compliant with ISO 17034 using authenticated strains from CECT®. These ready-to-use microbiological controls minimize the need for maintaining control strains in the test laboratory and guarantee the use of an authenticated control culture for every quality control test.

Vitroids are derived from a traceable culture obtained freeze-dried from CECT®.


Bacillus spizizenii WDCM 00003 Vitroids are used as reference strains in routine quality control for food, beverage, water, and environmental testing. Additionally, they are excellent for use in method development and validations, as starter cultures, and for use in research studies and educational purposes.

Features and Benefits

  • Ready-to-use at convenient CFU ranges
  • No dilution required
  • Quick rehydration in 10 minutes
  • 2-3 years of shelf life in a standard -20 °C freezer
  • Complete with a comprehensive certificate of analysis
  • No maintenance of stock cultures
  • No recovery time or pre-enrichment step is necessary
  • Robust, efficient, and reliable
  • Easy-to-use
  • Cost-effective

Other Notes

Strain History:

< NCIB < ATCC < N.R. Smith, NRS 231 < K.F. Kellerman
Strain origin: CECT® 356

Other cell culture collections:

  • DSM 347
  • ATCC® 6633
  • NCTC 10400
Click here to access BacDive database for more info on metabolism, genome, growth media and more

Legal Information

Derived from CECT® strains
ATCC is a registered trademark of American Type Culture Collection
CECT is a registered trademark of Universitat de Valencia
Vitroids is a trademark of Merck KGaA, Darmstadt, Germany

Storage Class

6.2 - Infectious substances



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