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04272

Sigma-Aldrich

Sodium phosphate dibasic dihydrate

meets analytical specification of Ph. Eur., BP, 98.5-101% (calc. to the dried substance)

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Synonym(s):
sec-Sodium phosphate, Disodium hydrogen phosphate dihydrate, Disodium phosphate, di-Sodium hydrogen phosphate dihydrate
Linear Formula:
Na2HPO4 · 2H2O
CAS Number:
Molecular Weight:
177.99
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

agency

meets analytical specifications of BP
meets analytical specifications of Ph. Eur.

assay

98.5-101% (calc. to the dried substance)

form

crystals

quality

meets analytical specification of Ph. Eur., BP

impurities

reducing matter, complies
residual solvents, complies
≤0.001% heavy metals (as Pb)
≤1% NaH2PO4 (calc. to dried subst.)

loss

19.5-21% loss on drying, 130 °C

pH

9.0-9.4 (20 °C, 1%)

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤100 mg/kg

cation traces

As: ≤2 mg/kg
Fe: ≤10 mg/kg

suitability

complies for appearance of solution

SMILES string

O.O.[Na+].[Na+].OP([O-])([O-])=O

InChI

1S/2Na.H3O4P.2H2O/c;;1-5(2,3)4;;/h;;(H3,1,2,3,4);2*1H2/q2*+1;;;/p-2

Inchi Key

KDQPSPMLNJTZAL-UHFFFAOYSA-L

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1.936221.932221.19753
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04272

Sodium phosphate dibasic dihydrate

Essential Grade
form

crystals

form

-

form

-

form

solid

assay

98.5-101% (calc. to the dried substance)

assay

-

assay

-

assay

≥99.0% (acidimetric)

cation traces

As: ≤2 mg/kg, Fe: ≤10 mg/kg

cation traces

-

cation traces

-

cation traces

-

anion traces

chloride (Cl-): ≤50 mg/kg, sulfate (SO42-): ≤100 mg/kg

anion traces

-

anion traces

-

anion traces

-

pH

9.0-9.4 (20 °C, 1%)

pH

-

pH

-

pH

9.0-9.4 (20 °C, 50 g/L in H2O)

application

Sodium phosphate dibasic dihydrate has been used for isolating DNA and RNA from bacterial samples of human gastrointestinal (GI) tract.

Biochem/physiol Actions

Sodium phosphate dibasic dihydrate is an important component of running buffer of denaturing gel electrophoresis.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Erwin G Zoetendal et al.
Nature protocols, 1(2), 954-959 (2007-04-05)
The human gastrointestinal (GI) tract contains a complex microbial community that consists of numerous uncultured microbes. Therefore, nucleic-acid-based approaches have been introduced to study microbial diversity and activity, and these depend on the proper isolation of DNA, rRNA and mRNA.
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