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Lipase A Candida antarctica immobilized on Immobead 150, recombinant from Aspergillus oryzae

≥500 U/g

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in Aspergillus oryzae

Quality Level

form

beads (powder like)
beads

specific activity

≥500 U/g

storage temp.

2-8°C

General description

Research area: Cellsignalling. Lipase A Candida Antarctica, CalA is a thermostable, calcium-dependent enzyme with high substrate specificity. CalA comprises of the catalytic triad (Ser184, Asp334, His366) and has an α/β hydrolase structural fold.

Application

Lipase A Candida antarctica immobilized on Immobead 150, recombinant from Aspergillus oryzae has been used in the synthesis of enantiopure (R)-salsolinol and adsorption kinetics studies using Quartz crystal microbalance with dissipation (QCM-D).It has also been used tostudy the esterification of difluorinated alcohols.
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biochem/physiol Actions

Lipase A Candida Antarctica, CalA is highly specific for alcohols and esterifies the trans-isomer of fatty acids. CalA recognizes highly branched acyl groups and is active on alcohols with steric hindrance. CalA catalyzes the production of enantiopure amino acids and aids in the synthesis of chiral cyanohydrins. It may find industrial applications for its thermostable functionality in paper industry.Lipase A Candida antarctica(CAL-A) shows acetyltransferase activity by synthesizing fatty acid esters from certain alcohols and natural oils in an aqueous environment.

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol butyric acid per minute at pH 10.0 and 40°C (tributyrin, Cat. No. 91010, as substrate)

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Karel Pomeisl et al.
Bioorganic & medicinal chemistry, 27(7), 1246-1253 (2019-02-20)
An enzymatic alternative to the chemical synthesis of chiral gem-difluorinated alcohols has been developed. The method is highly effective and stereoselective, feasible at laboratory temperature, avoiding the use of toxic heavy metal catalysts which is an important benefit in medicinal
The acyltransferase activity of lipase CAL-A allows efficient fatty acid esters formation from plant oil even in an aqueous environment
Muller J, et al.
European Journal of Lipid Science and Technology, 117(12), 1903-1907 (2015)
Biotechnological applications of Candida antarctica lipase A: State-of-the-art
de Maria PD, et al.
Journal of Molecular Catalysis. B, Enzymatic, 37(1-6), 36-46 (2005)
X-ray structure of Candida antarctica lipase A shows a novel lid structure and a likely mode of interfacial activation
Ericsson D, et al.
Journal of molecular biology, 376(1), 109-119 (2008)
Improved acylation of phytosterols catalyzed by Candida antarctica lipase A with superior catalytic activity
Panpipat W, et al.
Biochemical Engineering Journal, 70(1), 55-62 (2013)

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