MilliporeSigma
All Photos(1)

Documents

62310

Sigma-Aldrich

Lipase from Rhizopus niveus

powder (fine), ≥1.5 U/mg

Sign Into View Organizational & Contract Pricing

Synonym(s):
Triacylglycerol acylhydrolase, Triacylglycerol lipase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.26

biological source

fungus (Rhizopus niveus)

Quality Level

form

powder (fine)

specific activity

≥1.5 U/mg

mol wt

Mr ~83000

storage temp.

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
L8525L1754L4277
Sigma-Aldrich

62310

Lipase from Rhizopus niveus

Lipase from Candida rugosa lyophilized powder, ≥40,000 units/mg protein

L8525

Lipase from Candida rugosa

Lipase from Candida rugosa Type VII, ≥700 unit/mg solid

L1754

Lipase from Candida rugosa

Sigma-Aldrich

L4277

Lipase from Aspergillus oryzae

biological source

fungus (Rhizopus niveus)

biological source

fungus (Candida rugosa)

biological source

fungus (candida rugosa)

biological source

-

form

powder (fine)

form

lyophilized powder

form

lyophilized

form

lyophilized

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

mol wt

Mr ~83000

mol wt

-

mol wt

-

mol wt

-

Quality Level

100

Quality Level

-

Quality Level

-

Quality Level

-

General description

Lipases hydrolyze ester bonds of triacylglycerols and in some conditions, catalyze the synthesis of ester bonds by transesterification. Lipase from Rhizopus niveus exists as a single polypeptide chain with a molecular mass of about 30-34kDa.

Application

Lipase from Rhizopus niveus has been used to determine the positional distribution of fatty acids within triacylglycerol by hydrolysis of the acyl ester linkage at C-1 and C-3. It has also been used to induce intragastric lipolysis and study its effects on felodipine (a medication) release from a hydrophilic, extended release tablet.

Biochem/physiol Actions

Lipase from Rhizopus niveus is specifically used to produce cocoa butter substitutes.
Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol fatty acid from a triglyceride per minute at pH 7.7 and 40°C (olive oil as substrate)]; 300 U as described above are equivalent to ~1 U using triolein, Cat. No. 62314, at pH 8.0 and 40°C as substrate.

Other Notes

Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.
Substrate specificity: preferentially hydrolyzes the 1- and 3-positions(middle to long fatty acids); optimum pH 5.0-7.0; optimum temperature 30-45°C; Selective enzymatic removal of protecting functions (C-terminal COOH function)

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Purification, characterization and crystallization of two types of lipase from Rhizopus niveus
Mitsutaka Kohno
Bioscience, Biotechnology, and Biochemistry, 58(6) (1994)
Simulation of gastric lipolysis and prediction of felodipine release from a matrix tablet in the fed stomach
A.Diakidou
European Journal of Pharmaceutical Sciences (2009)
Y. H. Hui and George G. Khachatourians
Food Biotechnology (1995)
P. Braun et al.
Synlett, 105-105 (1990)
Analysis of triacylglycerol accumulation under nitrogen deprivation in the red alga Cyanidioschyzon merolae
Masakazu Toyoshima
Microbiology (2016)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service